Gleis Week 8 - Revision history

Gleis at 08:08, 18 October 2013

2013-10-18T08:08:39Z

2013-10-18T07:42:38Z

2013-10-18T07:32:08Z

2013-10-18T07:21:50Z

2013-10-18T07:20:41Z

2013-10-18T07:19:18Z

‎Lab Journal part 2

2013-10-18T07:02:21Z

‎Lab Journal part 2

2013-10-18T07:01:40Z

2013-10-18T05:46:46Z

2013-10-18T05:45:33Z

‎Lab Journal part 2

← Older revision		Revision as of 08:08, 18 October 2013
Line 1,004:		Line 1,004:
	47.Compared data in Excel to data in MAPPFinder		47.Compared data in Excel to data in MAPPFinder

−	48.	+	48.Comparisons were highlighted using a similar color scheme
		+	*[[Media:Decreased Expression Vibrio Gleis-Criterion1-GO-Criterion0-GO.xls]]
		+
		+	49.The expression of genes involved in catabolic processes decreased while expression of cell metabolic and cell cycle processes increased. This suggests that the patient-derived Vibrio is expending great amounts of energy and dividing rapidly while the lab-grown Vibrio is building itself up and reserving energy. From this information we can see that at a molecular level the patient-derived Vibrio appear to be much more aggressive in finding food and dividing while the lab-grown Vibrio seem to be suing food sources to build themselves up and divide at a slower rate
		+
		+	50.[[Media:Gleis - Merrell Compiled Raw Data Vibrio.gmf]]

@@ Line 992: / Line 992: @@
 .The results of the MAPPFinder were saved as: [[Media:Decreased Expression Vibrio Gleis-Criterion1-GO-Criterion0-GO.txt]]
-.
+.The txt file was opened in Excel and compared with my partners data
 ===Relevant Files===

@@ Line 990: / Line 990: @@
 .The MAPP file for this search is [[Media:Outer membrane-bounded periplasmic space.mapp]]
-.The results of the MAPPFinder were saved as: [[Media:Deacreased Expression Vibrio Gleis-Criterion1-GO.txt]]
+.The results of the MAPPFinder were saved as: [[Media:Decreased Expression Vibrio Gleis-Criterion1-GO-Criterion0-GO.txt]]
 .

← Older revision		Revision as of 07:21, 18 October 2013
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	43.The results of the MAPPFinder were saved as: [[Media:Deacreased Expression Vibrio Gleis-Criterion1-GO.txt]]		43.The results of the MAPPFinder were saved as: [[Media:Deacreased Expression Vibrio Gleis-Criterion1-GO.txt]]
		+
		+	44.

	===Relevant Files===		===Relevant Files===

@@ Line 990: / Line 990: @@
 .The MAPP file for this search is [[Media:Outer membrane-bounded periplasmic space.mapp]]
-.
+.The results of the MAPPFinder were saved as: [[Media:Deacreased Expression Vibrio Gleis-Criterion1-GO.txt]]
 ===Relevant Files===

← Older revision		Revision as of 07:19, 18 October 2013
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	34.My results corresponded with my partner Mitchell's results		34.My results corresponded with my partner Mitchell's results
		+
		+	35.Clicked on outer-membrane bounded periplasmic space under VCA0583
		+
		+	36.A results window opened
		+
		+	37.Searched uniport database with geneID of interest to determine expression from results of GO term search
		+
		+	38.VCA0583 is also known as Q9KM06_VIBCH and it showed little change in expression.
		+
		+	39.Clicked on the result box of Q9KM06_VIBCH
		+
		+	40.A new internet explorer window opened.
		+
		+	41.Clicked on the UniProt link and learned that the function of the protein is membrane transport
		+
		+	42.The MAPP file for this search is [[Media:Outer membrane-bounded periplasmic space.mapp]]
		+
		+	43.

	===Relevant Files===		===Relevant Files===

@@ Line 972: / Line 972: @@
 :*transporter activity
-.
+.My results corresponded with my partner Mitchell's results
 ===Relevant Files===
 [[Media:Deacreased Expression Vibrio Gleis-Criterion1-GO.txt]]

@@ Line 901: / Line 901: @@
 :*The terms are not the same as my partner Mitchell's.  This is likely because the newer dataset contained fewer errors and resulted in a greater number of genes processed by MAPPFinder.
-.
+.Entered Gene IDs mentioned by Merrell et al. (2002) into Gene ID search.
 ===Relevant Files===
 [[Media:Deacreased Expression Vibrio Gleis-Criterion1-GO.txt]]

@@ Line 880: / Line 880: @@
 .Checked boxes next to "Gene Ontology" and "p value"
-.Clicked the "Browse" button and saved the file as "UPLOAD"
+.Clicked the "Browse" button and saved the file as [[Media:Deacreased Expression Vibrio Gleis-Criterion1-GO.txt]]
 .Clicked "Run MAPPFinder"

@@ Line 866: / Line 866: @@
 .The file was saved as a (.gex) file
-:*[[Media:Gleis - Merrell Compiled Raw Data Vibrio.gex
+:*[[Media:Gleis - Merrell Compiled Raw Data Vibrio.gex]]
 .Launched the MAPPFinder program