HDelgadi Week 7 - Revision history

HDelgadi: signature and template added

2013-10-10T04:08:09Z

signature and template added

2013-10-10T03:11:10Z

answer to number 16

2013-10-10T03:03:58Z

answer to number 15

2013-10-10T02:59:31Z

answer to number 14

2013-10-10T02:56:15Z

answer to number 13

2013-10-10T02:52:38Z

answer to number 12

2013-10-10T02:39:20Z

Answer to number 11

2013-10-10T02:30:21Z

answer to number 10

2013-10-10T02:19:20Z

answered question 9

2013-10-10T02:06:52Z

added answer for number 7

← Older revision		Revision as of 04:08, 10 October 2013
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	16. (p. 121) What types of control spots would you like to see in this type of experiment? How could you verify that you had truly deleted or overexpressed a particular gene?		16. (p. 121) What types of control spots would you like to see in this type of experiment? How could you verify that you had truly deleted or overexpressed a particular gene?
	*I would like to see repressed control spots. I could verify the deletion or over-expression of a particular gene by looking at the consequential colored spots, so if they are bright red then there has been a great induction by a deletion or over-expression of a gene. Comparing the colored spots before and after the deletion or over-expression of a particular gene would help with the verification of the desired results.		*I would like to see repressed control spots. I could verify the deletion or over-expression of a particular gene by looking at the consequential colored spots, so if they are bright red then there has been a great induction by a deletion or over-expression of a gene. Comparing the colored spots before and after the deletion or over-expression of a particular gene would help with the verification of the desired results.
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		+	[[User:HDelgadi\|HDelgadi]] ([[User talk:HDelgadi\|talk]]) 21:08, 9 October 2013 (PDT)
		+	{{HDelgadi}}

← Older revision		Revision as of 03:11, 10 October 2013
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	16. (p. 121) What types of control spots would you like to see in this type of experiment? How could you verify that you had truly deleted or overexpressed a particular gene?		16. (p. 121) What types of control spots would you like to see in this type of experiment? How could you verify that you had truly deleted or overexpressed a particular gene?
		+	*I would like to see repressed control spots. I could verify the deletion or over-expression of a particular gene by looking at the consequential colored spots, so if they are bright red then there has been a great induction by a deletion or over-expression of a gene. Comparing the colored spots before and after the deletion or over-expression of a particular gene would help with the verification of the desired results.

← Older revision		Revision as of 03:03, 10 October 2013
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	15. (p. 121) Could the loss of a repressor or the overexpression of a transcription factor result in the repression of a particular gene?		15. (p. 121) Could the loss of a repressor or the overexpression of a transcription factor result in the repression of a particular gene?
		+	*The loss of a repressor or the over-expression of a transcription factor can result in the repression of a particular gene.

	16. (p. 121) What types of control spots would you like to see in this type of experiment? How could you verify that you had truly deleted or overexpressed a particular gene?		16. (p. 121) What types of control spots would you like to see in this type of experiment? How could you verify that you had truly deleted or overexpressed a particular gene?

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 . (p. 121) What color spots would you expect to see on the chip when the transcription factor Yap1p is overexpressed?
 . (p. 121) Could the loss of a repressor or the overexpression of a transcription factor result in the repression of a particular gene?
 . (p. 121) What types of control spots would you like to see in this type of experiment? How could you verify that you had truly deleted or overexpressed a particular gene?

← Older revision		Revision as of 02:56, 10 October 2013
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	13. (p. 121) Given rule one on page 109, what color would you see on a DNA chip when cells had their repressor gene TUP1 deleted?		13. (p. 121) Given rule one on page 109, what color would you see on a DNA chip when cells had their repressor gene TUP1 deleted?
		+	*The color would be bright red for a strong induction.

	14. (p. 121) What color spots would you expect to see on the chip when the transcription factor Yap1p is overexpressed?		14. (p. 121) What color spots would you expect to see on the chip when the transcription factor Yap1p is overexpressed?

← Older revision		Revision as of 02:52, 10 October 2013
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	12. (p. 120) What mechanism could the genome use to ensure genes for enzymes in a common pathway are induced or repressed simultaneously?		12. (p. 120) What mechanism could the genome use to ensure genes for enzymes in a common pathway are induced or repressed simultaneously?
		+	*The addition or depletion of glucose for example can cause genes that share a common pathway to be induced or repressed simultaneously if they share the same transcription factor binding sites.

	13. (p. 121) Given rule one on page 109, what color would you see on a DNA chip when cells had their repressor gene TUP1 deleted?		13. (p. 121) Given rule one on page 109, what color would you see on a DNA chip when cells had their repressor gene TUP1 deleted?

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 . (p. 120) Why would TCA cycle genes be induced if the glucose supply is running out?
+*The TCA cycle genes are induced by glucose since they would try to use up the depleting glucose due to its importance. Glucose turns into glycolysis and ultimately pyruvate which promotes the TCA cycle.
 . (p. 120) What mechanism could the genome use to ensure genes for enzymes in a common pathway are induced or repressed simultaneously?

← Older revision		Revision as of 02:19, 10 October 2013
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	9. (p. 118) Why would most spots be yellow at the first time point?		9. (p. 118) Why would most spots be yellow at the first time point?
		+	*An equal amount of genes are being repressed (green spots) and reduced (red spots); therefore yellow spots appear.

	10. (p. 118) Go to http://www.yeastgenome.org and search for the gene TEF4; you will see it is involved in translation. Look at the time point labeled OD 3.7 in Figure 4.12, and find the TEF4 spot. Over the course of this experiment, was TEF4 induced or repressed? Hypothesize why TEF4’s gene regulation was part of the cell’s response to a reduction in available glucose (i.e., the only available food).		10. (p. 118) Go to http://www.yeastgenome.org and search for the gene TEF4; you will see it is involved in translation. Look at the time point labeled OD 3.7 in Figure 4.12, and find the TEF4 spot. Over the course of this experiment, was TEF4 induced or repressed? Hypothesize why TEF4’s gene regulation was part of the cell’s response to a reduction in available glucose (i.e., the only available food).

← Older revision		Revision as of 02:06, 10 October 2013
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	7. (p. 110) Were any of the genes in Figure 4.7b transcribed similarly?		7. (p. 110) Were any of the genes in Figure 4.7b transcribed similarly?
		+	*I would say gene X and gene Y were transcribed similarly since their ratios increase by the third hour, decrease by the fifth hour, and decrease even more significantly by the ninth hour, so their transcription activity fluctuates similarly with the depletion of oxygen.

	9. (p. 118) Why would most spots be yellow at the first time point?		9. (p. 118) Why would most spots be yellow at the first time point?