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Revision as of 21:49, 19 October 2013

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*#Number of probes that met the [Avg_LogFC_All]>0.25 AND [PValue]<0.05 criteria

*#Number of probes in the dataset

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*: The other results were all different. This makes sense because my partner used an older version of the Vibrio Gene Database. The similar terms were the ones linked to Uniprot IDs and Go terms, but the other information was more updated in my dataset. There were larger results in my procedure because there were more links to the genes made between the times the data was uploaded.

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*: The other results were all different. This makes sense because my partner used an older version of the Vibrio Gene Database. The similar terms were the ones linked to Uniprot IDs and Go terms, but the other information was more updated in my dataset. There were larger results in my procedure because there were more links to the genes made between the times the data was uploaded.

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*Interpreting the GO terms. Focusing on relatedness

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*:The main reoccurring term I found was flagellum assembly and Pilus assembly. These were found under cellular component organization, biogenisis at cellular level, and cellular process under multiple sub branches. Although there were other differences in gene expression as well, I thought that these were particular significant.By looking into the gene exprssion differences between the pathogenic vs lab I found that many of them had increased activity in the Pathogenic strain. This led me to conclude that the structure of the flagella and pili is probably altered in the pathogenic strain in a way that makes it harmful. It is possible the the change in pili structure makes it so that it is more likely to transfer mutagenic plasmid DNA to other cells causing them to become pathogenic. The flagella structure could also have changed in this strain and played a role in its virulence. Another significant change in gene expression in the two was that the pathogenic strain had decreased mRNA degredation, which could have have alerted the number of mutations, contributing to the pathogenicity.

Vkuehn Week 8 - Revision history

Vkuehn: Finished!

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 *#Number of probes that met the [Avg_LogFC_All]>0.25 AND [PValue]<0.05 criteria
 *#Number of probes in the dataset
 *: The other results were all different. This makes sense because my partner used an older version of the Vibrio Gene Database. The similar terms were the ones linked to Uniprot IDs and Go terms, but the other information was more updated in my dataset. There were larger results in my procedure because there were more links to the genes made between the times the data was uploaded.