Difference between revisions of "Vkuehn Week 2"

Revision as of 07:49, 6 September 2013

Individual Journal Assignment Week 2

5’-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta-3’
3'-gcatacgattatggtacaaggcgcatattgggtcggcggtcaaggcgaccgccgtaaaat-5'

mRNA:

5’-cguaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuua-3’

All possible reading frames translated:

+1: n-ter   R-M-L-I-P-C-S-A-T-N-P-A-A-S-S-A-G-G-I-L   c-ter
+2: n-ter   V-C   c-ter
+3: n-ter   Y-A-N-T-M-F-R-V-L-P-S-R-Q-F-R-W-R-H-F   c-ter

Bottom strand mRNA:

 3'-gcauacgauuaugguacaaggcgcauauugggucggcggucaaggcgaccgccguaaaau-5'

Possible reading frames translated:

-1: n-ter   stop
-2: n-ter   K-M-P-P-A-E-L-A-A--L-Y-A-E-H-G-I-S-I   c-ter
-3: n-ter   K-C-R-Q-R-N-W-R-L-G-Y-T-R-T-M-V-L-A-Y   c-ter

The following are open reading frames because they do not contain a stop codon: +1, +3, -2, -3

@@ Line 16: / Line 16: @@
 Possible reading frames translated:
-  -1: A-Y-D-Y-G-T-R-R-I-L-G-R-S-R-R-P
+  -1: n-ter   stop
- -2:
+ -2: n-ter   K-M-P-P-A-E-L-A-A--L-Y-A-E-H-G-I-S-I   c-ter
- -3:
+ -3: n-ter   K-C-R-Q-R-N-W-R-L-G-Y-T-R-T-M-V-L-A-Y   c-ter
+The following are open reading frames because they do not contain a stop codon:
++1, +3, -2, -3