Difference between revisions of "Kmeilak Week 2"

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(edited errors from last week's homework)
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==Week 2 Individual Journal Assignment==
 
==Week 2 Individual Journal Assignment==
  
 +
Initial sequence
 +
5'-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta-3'
 +
 +
Complementary sequence
 
  3'-gcatacgattatggtacaaggcgcatattgggtcggcggtcaaggcgaccgccgtaaaat-5'
 
  3'-gcatacgattatggtacaaggcgcatattgggtcggcggtcaaggcgaccgccgtaaaat-5'
  
  
*+1 strand: nonsense strand, first codon is stop codon.
+
*+1 strand: R-M-L-I-P-C-S-A-Y-N-P-A-A-S-S-A-G-G-I-L
*+2 strand: M-P-P-A-E-L-A-A-G-L-T-A-E-H-G-I-S-H-T
+
*+2 strand: V-C-stop-Y-H-V-P-R-I-T-Q-P-P-V-P-L-A-A-F
*+3 strand: L-C-R-Q-R-N-W-R-L-G-Y-T-R-N-M-V-L-A-Y (note: strand does not begin with Methionine, cannot exist)
+
*+3 strand: Y-A-N-T-M-F-R-V-stop-P-S-R-Q-F-R-W-R-H-F
*-1 strand: M-L-I-P-C-S-A-T-A-P-A-A-V-P-A-G-G-I-L
+
*-1 strand: stop-N-A-A-S-G-T-G-G-W-V-I-R-G-T-W-Y-stop-H-T
*-2 strand: V-L-stop (nonsense strand, does not begin with Methionine and terminates two amino acids in)
+
*-2 strand: K-M-P-P-A-E-L-A-A-G-L-Y-A-E-H-G-I-S-I
*-3 strand: M-L-I-P-C-S-A-Y-N-H-P-P-V-P-W-P-H-F
+
*-3 strand: K-C-R-Q-R-N-W-R-L-G-Y-T-R-N-M-V-L-A-Y
  
  
the +1, -3, and -1 reading frames are open reading frames.
+
the +1, -2, and -3 reading frames are open reading frames.

Revision as of 04:38, 11 September 2013

Week 2 Individual Journal Assignment

Initial sequence

5'-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta-3'

Complementary sequence

3'-gcatacgattatggtacaaggcgcatattgggtcggcggtcaaggcgaccgccgtaaaat-5'


  • +1 strand: R-M-L-I-P-C-S-A-Y-N-P-A-A-S-S-A-G-G-I-L
  • +2 strand: V-C-stop-Y-H-V-P-R-I-T-Q-P-P-V-P-L-A-A-F
  • +3 strand: Y-A-N-T-M-F-R-V-stop-P-S-R-Q-F-R-W-R-H-F
  • -1 strand: stop-N-A-A-S-G-T-G-G-W-V-I-R-G-T-W-Y-stop-H-T
  • -2 strand: K-M-P-P-A-E-L-A-A-G-L-Y-A-E-H-G-I-S-I
  • -3 strand: K-C-R-Q-R-N-W-R-L-G-Y-T-R-N-M-V-L-A-Y


the +1, -2, and -3 reading frames are open reading frames.

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