Difference between revisions of "Stephen Louie Week 2"

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(Reorganized strands under genetic code heading)
(Added corrections to the negative reading frames)
 
(14 intermediate revisions by one user not shown)
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  3'-GCATACGATTATGGTACAAGGCGCATATTGGGTCGGCGGTCAAGGCGACCGCCGTAAAAT-5'
 
  3'-GCATACGATTATGGTACAAGGCGCATATTGGGTCGGCGGTCAAGGCGACCGCCGTAAAAT-5'
 +
 +
 +
==Possible Reading Frames==
 +
 +
====+1====
 +
 +
A-Y-D-Y-G-T-R-R-I-L-G-R-R-S-R-R-P-P-Stop
 +
 +
'''R M L I P C S A Y N P A A S S A G G I L'''
 +
 +
====+2====
 +
 +
H-T-I-M-V-Q-G-A-Y-W-V-G-G-Q-G-D-R-R-K
 +
 +
'''V C Stop Y H V P R I T Q P P V P L A A F'''
 +
 +
====+3====
 +
 +
I-R-L-W-Y-K-A-H-I-G-S-A-V-K-A-T-A-V-K
 +
 +
'''Y A N T M F R V Stop P S R Q F R W R H F'''
 +
 +
====-1====
 +
 +
I-L-R-R-S-P-STOP
 +
 +
'''Stop N A A S G T G G W V I R G T W Y Stop H T'''
 +
 +
====-2====
 +
 +
F-Y-G-G-R-L-D-R-R-P-N-M-R-L-V-P-Stop
 +
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'''K M P P A E L A A G L Y A E H G I S I'''
 +
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====-3====
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 +
F-T-A-V-A-L-T-A-D-P-I-C-A-L-Y-H-N-V-M
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'''K C R Q R N W R L G Y T R N M V L A Y'''
 +
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==Note==
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+2,+3,-3 are open reading frames
 +
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'''+1, -2 and -3 are open reading frames'''
 +
 +
==Comments==
 +
 +
The reason for my mistakes on Reading Frames +1, +2, and +3 was difficult to discern initially.  At first I was wondering whether it had to do with a mistake within the sequence.  I realized that my process of translation was incorrect.  When I was translating, I would convert the coding nucleotides into their complements, then look for the matching codon.  In essence, I had the -1 reading frame of the complementary strand when I thought I was getting the +1 reading frame for the coding strand.
 +
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==Other links==
 +
{{Slouie}}
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 +
[[Category:Journal Entry]]

Latest revision as of 23:46, 12 September 2013

Contents

[edit] Genetic Code

[edit] Coding Strand

5’-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta-3’

[edit] Complementary Strand

3'-GCATACGATTATGGTACAAGGCGCATATTGGGTCGGCGGTCAAGGCGACCGCCGTAAAAT-5'


[edit] Possible Reading Frames

[edit] +1

A-Y-D-Y-G-T-R-R-I-L-G-R-R-S-R-R-P-P-Stop

R M L I P C S A Y N P A A S S A G G I L

[edit] +2

H-T-I-M-V-Q-G-A-Y-W-V-G-G-Q-G-D-R-R-K

V C Stop Y H V P R I T Q P P V P L A A F

[edit] +3

I-R-L-W-Y-K-A-H-I-G-S-A-V-K-A-T-A-V-K

Y A N T M F R V Stop P S R Q F R W R H F

[edit] -1

I-L-R-R-S-P-STOP

Stop N A A S G T G G W V I R G T W Y Stop H T

[edit] -2

F-Y-G-G-R-L-D-R-R-P-N-M-R-L-V-P-Stop

K M P P A E L A A G L Y A E H G I S I

[edit] -3

F-T-A-V-A-L-T-A-D-P-I-C-A-L-Y-H-N-V-M

K C R Q R N W R L G Y T R N M V L A Y

[edit] Note

+2,+3,-3 are open reading frames

+1, -2 and -3 are open reading frames

[edit] Comments

The reason for my mistakes on Reading Frames +1, +2, and +3 was difficult to discern initially. At first I was wondering whether it had to do with a mistake within the sequence. I realized that my process of translation was incorrect. When I was translating, I would convert the coding nucleotides into their complements, then look for the matching codon. In essence, I had the -1 reading frame of the complementary strand when I thought I was getting the +1 reading frame for the coding strand.

[edit] Other links

Retrieved from "https://xmlpipedb.lmucs.io/biodb/fall2013/index.php?title=Stephen_Louie_Week_2&oldid=1499"
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