Difference between revisions of "KSherbina Week 3"

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(Reading Frames: Corrected commands to translate -1, -2, and -3 reading frames.)
(Inputted response to first question in the XMLPipeDB Match Practice.)
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*the -1 reading frame:
 
*the -1 reading frame:
 
  cat prokaryote.txt | sed "y/atcg/tagc/" | rev | sed "s/^..//g" | sed "s/.../& /g" | sed "s/t/u/g" | sed -f genetic-code.sed
 
  cat prokaryote.txt | sed "y/atcg/tagc/" | rev | sed "s/^..//g" | sed "s/.../& /g" | sed "s/t/u/g" | sed -f genetic-code.sed
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 +
===XMLPipeDB Match Practice===
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 +
#There are two unique matches: go:0009165 and go:0009165. The pattern go:009165 appears twice and the pattern go:009165 appears once in the file. These patterns represent a gene ontology term, which describes what biological function the product of a gene is involved in.
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#
  
  

Revision as of 06:05, 13 September 2013

Katrina Sherbina
Class Page    User Page
Assignment Description Week 1 Week 2 Week 3 Week 4 Week 5 Week 6 Week 7 Week 8 Week 9 Week 10 Week 11 Week 12 Week 13 Week 15
Class Journal Week 1 Week 2 Week 3 Week 4 Week 5 Week 6 Week 7 Week 8 Week 9
Individual Journal Week 2 Week 3 Week 4 Week 5 Week 6 Week 7 Week 8 Week 9 Week 10 Week 11
Other Week 5: Database Wiki
Final Project Team H(oo)KD Project Page Journal Club Presentation Project Individual Journal

Contents

The Genetic Code, by Computer

Complement of a Strand

To find the complementary strand of the sequence in the file prokaryote.txt, the following sequence of piped text was executed

cat prokaryote.txt | sed "y/atcg/tagc/"

Reading Frames

The following piped sequence of text was invoked to determine the sequence of amino acids encoded by

  • the +1 reading frame:
cat prokaryote.txt | sed "s/.../& /g" | sed "s/t/u/g" | sed -f genetic-code.sed
  • the +2 reading frame:
cat prokaryote.txt | sed "s/^.//g" | sed "s/.../& /g" | sed "s/t/u/g" | sed -f genetic-code.sed
  • the +3 reading frame:
cat prokaryote.txt | sed "s/^..//g" | sed "s/.../& /g" | sed "s/t/u/g" | sed -f genetic-code.sed
  • the -1 reading frame:
cat prokaryote.txt | sed "y/atcg/tagc/" | rev | sed "s/.../& /g" | sed "s/t/u/g" | sed -f genetic-code.sed
  • the -2 reading frame:
cat prokaryote.txt | sed "y/atcg/tagc/" | rev | sed "s/^.//g" | sed "s/.../& /g" | sed "s/t/u/g" | sed -f genetic-code.sed
  • the -1 reading frame:
cat prokaryote.txt | sed "y/atcg/tagc/" | rev | sed "s/^..//g" | sed "s/.../& /g" | sed "s/t/u/g" | sed -f genetic-code.sed

XMLPipeDB Match Practice

  1. There are two unique matches: go:0009165 and go:0009165. The pattern go:009165 appears twice and the pattern go:009165 appears once in the file. These patterns represent a gene ontology term, which describes what biological function the product of a gene is involved in.


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