Difference between revisions of "Kmeilak Week 4"

Latest revision as of 04:40, 20 September 2013

[edit] Week 4

[edit] Transcription and Translation “Taken to the Next Level”

[edit] 1. code

cat infA-E.coli-K12.txt  | sed "s/cat[at]at/ <minus10box> & <\/minus10box> /g" | sed -r "s/.{17} <minus10box>/ <\/minus35box> &/g" | sed -r "s/.{6} <\/minus35box>/ <minus35box> &/g" | sed -r "s/ <minus10box> .{6} <\/minus10box> .{5}/& <tss> /g" | sed "s/ <tss> ./& <\/tss> /g" | sed "s/gagg/ <rbs> & <\/rbs>\n/g" | sed "2s/atg/ <start_codon> & <\/start_codon> /1" | sed -r "2s/<\/start_codon> ...*t[ag][ag]/& <\/stop_codon> /g" | sed "2s/... <\/stop_codon>/ <stop_codon> &/g" | sed "2s/aaaaggt/ <terminator> &/g" | sed "2s/tttt..../& <\/terminator> /2"

• sequence of -35 box

tttact

• sequence of -10 box

cattat

• transcription start site base pair

c

• sequence of ribosome binding site

gagg

• start codon

atg

• stop codon

tga

• terminator sequence

aaaaggtcggtttaaccggcctttttatt

[edit] 2. mRNA sequence

gccaaguuuaaugccaucacuauggggucuccuaaucuaccgguuucuucuguuauaacuuuacguuccauggcaagaacuuugcaacggauuaugguacaaggcgcaucucaaucuuuugccagugcaccaaugacguguguagaggccauuuuacgcguuuuugauguaggcguaggacugcccgcuguuucacugacaacuugacuggggcaugcuggacucguuuccggcguaacagaaggcaucagcgacuaacaaaauggcggacuacccgcuucucuuucuugcucauuuuccagccaaauuggccggaaaaauaa

[edit] 3. Amino Acid sequence

M G S P N L P V S S V I T L R S M A R T L Q R I M V Q G A S Q S F A S A P M T C V E A I L R V F D V G V G L P A V S L T T

• Main Page
• Kevin's User Page
• Week 1
• Week 2
• Week 3
• Week 4
• Week 5
• Week 6
• Week 7
• Week 8
• Week 9
• Week 10
• Week 11
• Week 12
• Week 13
• Week 14
• Week 15
• Class Journal Week 1
• Class Journal Week 2
• Class Journal Week 3
• Class Journal Week 4
• Class Journal Week 5
• Class Journal Week 6
• Class Journal Week 7
• Class Journal Week 8
• Class Journal Week 9
• Class Journal Week 10
• Class Journal Week 11
• Class Journal Week 12
• Class Journal Week 13
• Class Journal Week 14
• Class Journal Week 15
• Kmeilak Week 2
• Kmeilak Week 3
• Kmeilak Week 4
• Kmeilak Week 5
• Kmeilak Week 6
• Kmeilak Week 7
• Kmeilak Week 8
• Kmeilak Week 9
• Kmeilak Week 10
• Kmeilak Week 11
• Kmeilak Week 12
• Kmeilak Week 13
• Kmeilak Week 14
• Kmeilak Week 15

Kmeilak (talk) 21:20, 12 September 2013 (PDT)