Difference between revisions of "Gleis Week 7"

Revision as of 01:03, 10 October 2013

Introduction to MicroArrays

5.

• series 1=DIT1 gene
• series 2=SPS100 gene

6b. In format (gene,color at 1hr,color at 3hr, color at 5hr, color at 9hr)

(X,black,dim red,black,medium green)

(Y,black,medium red,dim green,bright green)

(Z,blak,dim red,dim red,dim red)

7. Gene X and Gene Y both exhibited an induction in transcription followed by a repression of transcription. Gene Z showed continuous induction of expression.

9. Most spots would be yellow at the first time point because there was little change in the percent of oxygen given to the cells. As a result there would be no reason for the cells to change levels of expression.

10. Over the course of the experiment TEF4 expression was repressed.TEF4 expression was repressed because available glucose declined. The decline of the cells energy caused the cell to repress expression of any genes that would result in the occurrence of high energy reactions. The TEF4 gene is involved in the energy intensive process of translational and was therefore down regulated in the absence of glucose.

11. Genes involved in the TCA cycle would be induced to produce as much energy the cell can while glucose is still available.

12.