Difference between revisions of "Ksherbina Week 7"
(→Answers to Discovery Questions from Chapter 4 of Discovering Genomics, Proteomics, and Bioinformatics: Altered question 6b Gene Y 3 hour and Gene Y 5 hour. Modified answer to question 9.) |
(→Answers to Discovery Questions from Chapter 4 of Discovering Genomics, Proteomics, and Bioinformatics: Added answer to #10.) |
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'''9.''' At the first time point, the cell density is very low. Consequently, there is no appreciable depletion of the source of glucose. Since there is no stress present, as there would be if the availability of glucose was significantly decreasing, most of the genes are not being induced or repressed. Thus, most of the spots are yellow. | '''9.''' At the first time point, the cell density is very low. Consequently, there is no appreciable depletion of the source of glucose. Since there is no stress present, as there would be if the availability of glucose was significantly decreasing, most of the genes are not being induced or repressed. Thus, most of the spots are yellow. | ||
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| + | '''10.''' As the experiment progresses, the processes of glycolysis, the TCA cycle, and oxidative phosphorylation likely slowed due to the depletion of glucose. As a result, ATP production through cell respiration decreased. Since a cell needs ATP to perform translation, the depletion of glucose inhibited translation. Therefore, over the course of the experiment, TEF4 was repressed. | ||
[[Category:Journal Entry]] | [[Category:Journal Entry]] | ||
[[Category:Individual Homework]] | [[Category:Individual Homework]] | ||
Revision as of 03:23, 10 October 2013
| Assignment Description | Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 15 |
| Class Journal | Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | |||||
| Individual Journal | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 |
| Other | Week 5: Database Wiki |
| Final Project | Team H(oo)KD Project Page | Journal Club Presentation | Project Individual Journal |
Answers to Discovery Questions from Chapter 4 of Discovering Genomics, Proteomics, and Bioinformatics
6b. Gene X 1 hour: black
- Gene X 3 hour: dim red
- Gene X 5 hour: black
- Gene X 9 hour: medium green
- Gene Y 1 hour: black
- Gene Y 3 hour: medium red
- Gene Y 5 hour: dim green (almost black)
- Gene Y 9 hour: bright green
- Gene Z 1 hour: black
- Gene Z 3 hour: dim red
- Gene Z 5 hour: dim red
- Gene Z 8 hour: dim red
7. Gene X and Gene Y were transcribed similarly. The genes were induced after 3 hours, went back to the control state (no fold change) after 5 hours, and were repressed after 9 hours.
9. At the first time point, the cell density is very low. Consequently, there is no appreciable depletion of the source of glucose. Since there is no stress present, as there would be if the availability of glucose was significantly decreasing, most of the genes are not being induced or repressed. Thus, most of the spots are yellow.
10. As the experiment progresses, the processes of glycolysis, the TCA cycle, and oxidative phosphorylation likely slowed due to the depletion of glucose. As a result, ATP production through cell respiration decreased. Since a cell needs ATP to perform translation, the depletion of glucose inhibited translation. Therefore, over the course of the experiment, TEF4 was repressed.
