'''6b. (p. 110) Look at Figure 4.7, which depicts the loss of oxygen over time and the transcriptional response of three genes. These data are the ratios of transcription for genes X, Y, and Z during the depletion of oxygen. Using the color scale from Figure 4.6 (bright, medium, dim green, black, dim, medium, or bright red), determine the color for each ratio in Figure 4.7b.'''

'''6b. (p. 110) Look at Figure 4.7, which depicts the loss of oxygen over time and the transcriptional response of three genes. These data are the ratios of transcription for genes X, Y, and Z during the depletion of oxygen. Using the color scale from Figure 4.6 (bright, medium, dim green, black, dim, medium, or bright red), determine the color for each ratio in Figure 4.7b.'''<br>

Gene X:

Gene X:<br>

1 hr- black

1 hr- black<br>

3 hr- red

3 hr- red<br>

5 hr- black

5 hr- black<br>

9 hr- green

9 hr- green<br><br>

Gene Y:

Gene Y:<br>

1 hr- black  

1 hr- black <br>

3 hr- red  

3 hr- red <br>

5 hr- green

5 hr- green<br>

9 hr- green

9 hr- green<br><br>

Gene Z:

Gene Z:<br>

1 hr- black

1 hr- black<br>

3 hr- red

3 hr- red<br>

5 hr- red

5 hr- red<br>

9 hr- red

9 hr- red<br><br>

'''7. (p. 110) Were any of the genes in Figure 4.7b transcribed similarly?'''

'''7. (p. 110) Were any of the genes in Figure 4.7b transcribed similarly?'''<br>

Gene X and Y were transcribed similarly. They were both induced hour 3 and repressed hours 5 and 9.  

Gene X and Y were transcribed similarly. They were both induced hour 3 and repressed hours 5 and 9. <br><br>

'''9. (p. 118) Why would most spots be yellow at the first time point?'''

'''9. (p. 118) Why would most spots be yellow at the first time point?'''<br>

At the first time point, no change has yet occurred. Rapid change does not happen until later in time.

At the first time point, no change has yet occurred. Rapid change does not happen until later in time.<br><br>

'''10. (p. 118) Go to http://www.yeastgenome.org and search for the gene TEF4; you will see it is involved in translation. Look at the time point labeled OD 3.7 in Figure 4.12, and find the TEF4 spot. Over the course of this experiment, was TEF4 induced or repressed? Hypothesize why TEF4’s gene regulation was part of the cell’s response to a reduction in available glucose (i.e., the only available food).'''

'''10. (p. 118) Go to http://www.yeastgenome.org and search for the gene TEF4; you will see it is involved in translation. Look at the time point labeled OD 3.7 in Figure 4.12, and find the TEF4 spot. Over the course of this experiment, was TEF4 induced or repressed? Hypothesize why TEF4’s gene regulation was part of the cell’s response to a reduction in available glucose (i.e., the only available food).'''<br>