Difference between revisions of "Gleis Week 11"

Revision as of 22:53, 10 November 2013

GO Terms

Metacyclic: produced in an intermediate host, and infective for the definitive host; said of the infective stages of trypanosomes. http://medical-dictionary.thefreedictionary.com/metacyclic

Promastigote:The flagellate stage of a trypanosomatid protozoan, as that of any of the Leishmania parasites http://medical-dictionary.thefreedictionary.com/promastigote

Phagolysosome:a cytoplasmic body formed by the fusion of a phagosome, or ingested particle, with a lysosome containing hydrolytic enzymes. The enzymes digest most of the material within the phagosome http://medical-dictionary.thefreedictionary.com/phagolysosome

Tripartite:having three corresponding parts or copies http://www.merriam-webster.com/dictionary/tripartite

"Repeated-repeat": repeated tandem repeats, Copies of DNA sequences which lie adjacent to each other in the same orientation (direct tandem repeats) or in the opposite direction to each other http://www.nlm.nih.gov/cgi/mesh/2011/MB_cgi?mode=&term=Tandem+Repeat

bacterial clade:Related organisms descended from a common ancestor. For example, isolate M of HIV-1 (the human immunodeficiency virus) consists of at least ten clades. Imported from the Greek, klados, branch in 1911 in reference to the Tree of Life. http://www.medterms.com/script/main/art.asp?articlekey=22233

filarial:related to or infested with or transmitting parasitic worms especially filaria http://wordnetweb.princeton.edu/perl/webwn?s=filarial

trypanosomatid:common name for a member of the family Trypanosomatidae http://medical-dictionary.thefreedictionary.com/trypanosomatid

chromodomain:Chromodomains are highly conserved sequence motifs that have been identified in a variety of animal and plant species. A chromodomain harbours a methylated lysine residue. http://www.springerreference.com/docs/html/chapterdbid/34668.html

bromodomain:Protein containing at least one bromodomain. The bromodomain is a conserved region, approximately 70 amino acids, characteristic for a class of regulatory proteins. It mediates interactions with proteins that are necessary for transcriptional activation. http://www.uniprot.org/keywords/KW-0103

Reference Article Outline

I. Abstract

• Leishmania major causes leishmaniasis, a dreadful human disease found throughout the world.
• Many genes currently studied involved the pathogenic nature of Leishmania major
• The lack of certain genes in the genome of Leishmania suggest novel expression and regulation systems

II. Introduction

• Leishmania parasites are transmitted by sand flies
• Once Leishmania have infected the host, the parasite subsequently proliferates and spreads throughout the host.
• Different Leishmania species exhibit differences in genome composition and structure
• This article presents the structure and content of the Leishmania major genome
• Special emphasis is given to fundamental molecular processes and pathogen-speicific functions

III. Genome structure and content

• Shotgun sequencing was used to sequence the genome chromosome by chromosome
• Table 1 is a summary of genome information for Leishmania. It includes size and gene information
• Table 2 includes information regarding families of genes in the Leishmania genome
• Several algorithms were used to predict the number of genes of each type in the genome
• Many genes in Leishmania major resemble genes other Leishmania species
• Some genes appear to be Leishmania major specific
• The Leishmania genome contains genes for MIFs which are likely responsible for the promotion of parasite survival in the host.