Difference between revisions of "Kevinmcgee Week 11"
From LMU BioDB 2013
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glycoinositol-phospholipids | glycoinositol-phospholipids | ||
prenylation | prenylation | ||
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| + | ==Leishmania Background== | ||
| + | #Leishmania Major is a tropical parasite | ||
| + | #Sepctrum of disease, “Leishmaniases” | ||
| + | #*broad term describing a flesh eating virus specific to Leishmania | ||
| + | #*2 million infections in 88 countries annually | ||
| + | #Have adapted to avoid host destruction | ||
| + | #*curing is very hard and doesn’t always work | ||
| + | ==Genome Structure and Content== | ||
| + | #32,816,678 base pairs obtained | ||
| + | #36 chromosomes | ||
| + | #*single continuous sequence generated for each chromosome | ||
| + | #911 RNA genes | ||
| + | #*Organized differently in Tritryp genomes from L. Major | ||
| + | #8272 protein coding genes | ||
| + | #663 related families | ||
| + | #*Smaller gene families arose from gene duplication | ||
| + | #*Larger families have single genes at multiple locations on the gene | ||
| + | ==Genome Comparison== | ||
| + | #Leishmania is compared with other organisms | ||
| + | #*Trypanosoma Brucei | ||
| + | #*Trypanosoma Cruzi | ||
| + | #*Leishmania has many orthologs under in these genomes | ||
| + | #910 genes not orthologs | ||
| + | #*“Leishmania Restricted genes” | ||
| + | #**responsible for key metabolic differences | ||
| + | #LmjF33.1740 and LmjF33.1750 | ||
| + | #*Macrophage migration inhibition factor | ||
| + | #**Similar to that in humans that deals with immunity from macrophage | ||
| + | ==Significant Genetic Findings== | ||
| + | #Transcription | ||
| + | #*L. major genome is organized into 133 clusters of tens to hundreds of protein-coding genes on same DNA strand | ||
| + | #*Pollycistronic transcription initiates in both directions: | ||
| + | #**In divergent strand-switch regions | ||
| + | #**terminates within the convergent strand-switch regions | ||
| + | #*RNAP I, II and III were found in Trytrip | ||
| + | #**Very different from other eukaryotes | ||
| + | #*Not many other homologs of RNAP were found | ||
| + | #*These findings, along with the polycistronic gene organization, are consistent with posttranscriptional control mechanisms being the primary determinants of Tritryp gene expression | ||
| + | #RNA Processing | ||
| + | #*Polyadenylation is determined by trans-splicing of downstream mRNA | ||
| + | |||
| + | ==Implications== | ||
Revision as of 04:47, 12 November 2013
trypanosomatid Ecotins chymotrypsin amastins Sphingolipids PG-galactosyltransferases pseudogenes ribonuclease glycoinositol-phospholipids prenylation
Contents |
Leishmania Background
- Leishmania Major is a tropical parasite
- Sepctrum of disease, “Leishmaniases”
- broad term describing a flesh eating virus specific to Leishmania
- 2 million infections in 88 countries annually
- Have adapted to avoid host destruction
- curing is very hard and doesn’t always work
Genome Structure and Content
- 32,816,678 base pairs obtained
- 36 chromosomes
- single continuous sequence generated for each chromosome
- 911 RNA genes
- Organized differently in Tritryp genomes from L. Major
- 8272 protein coding genes
- 663 related families
- Smaller gene families arose from gene duplication
- Larger families have single genes at multiple locations on the gene
Genome Comparison
- Leishmania is compared with other organisms
- Trypanosoma Brucei
- Trypanosoma Cruzi
- Leishmania has many orthologs under in these genomes
- 910 genes not orthologs
- “Leishmania Restricted genes”
- responsible for key metabolic differences
- “Leishmania Restricted genes”
- LmjF33.1740 and LmjF33.1750
- Macrophage migration inhibition factor
- Similar to that in humans that deals with immunity from macrophage
- Macrophage migration inhibition factor
Significant Genetic Findings
- Transcription
- L. major genome is organized into 133 clusters of tens to hundreds of protein-coding genes on same DNA strand
- Pollycistronic transcription initiates in both directions:
- In divergent strand-switch regions
- terminates within the convergent strand-switch regions
- RNAP I, II and III were found in Trytrip
- Very different from other eukaryotes
- Not many other homologs of RNAP were found
- These findings, along with the polycistronic gene organization, are consistent with posttranscriptional control mechanisms being the primary determinants of Tritryp gene expression
- RNA Processing
- Polyadenylation is determined by trans-splicing of downstream mRNA
