Difference between revisions of "Ajvree Week 2"

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(added -1 strand)
(remaining strands, open frames, signature and template)
 
Line 12: Line 12:
  
 
  -1 strand:  STOP-N-A-A-S-G-T-G-G-W-V-I-R-G-T-W-STOP-H-T
 
  -1 strand:  STOP-N-A-A-S-G-T-G-G-W-V-I-R-G-T-W-STOP-H-T
  -2 strand:   
+
  -2 strand:  K-M-P-P-A-E-L-A-A-G-L-Y-A-E-H-G-I-S-I
 +
-3 strand:  K-C-R-Q-R-N-W-R-L-G-Y-T-R-N-M-V-L-A-Y
  
** In RNA, the T becomes a U, so everywhere you see a T in the sequence, read it as a U.
+
Open reading frames: Strands +1, -2, and -3
** The genetic code is read in the 5’ to 3’ direction.
+
 
** Use the single-letter abbreviations for the amino acids because that is what is commonly used by computer programs.
+
[[User:Ajvree|Ajvree]] ([[User talk:Ajvree|talk]]) 09:34, 6 September 2013 (PDT)
* Wiki Hint:  if you start your line with a single space character, it will appear in the grey box surrounded by the dashed line as shown above.
+
 
* Which of the reading frames (if any) of the reading frames you translated is an ''open reading frame'', i.e., does not contain a stop codon?
+
{{Ajvree}}
** By convention, the top strand frames are called +1, +2, +3, reading 5' to 3' and the bottom strand frames are called -1, -2, -3, reading 5' to 3'.
+

Latest revision as of 16:34, 6 September 2013

  • Write out the complementary strand of DNA below the strand shown and be sure to label the 5’ and 3’ ends of the complementary strand.
5’-cgt-atg-cta-ata-cca-tgt-tcc-gcg-tat-aac-cca-gcc-gcc-agt-tcc-gct-ggc-ggc-att-tta-3’
3'-gca-tac-gat-tat-ggt-aca-agg-cgc-ata-ttg-ggt-cgg-cgg-tca-agg-cga-ccg-ccg-taa-aat-5'
  • There are six possible reading frames in double-stranded DNA. Using the genetic code, translate all possible reading frames of this DNA sequence, keeping in mind the following rules.
+1 strand:  R-M-L-I-P-C-S-A-Y-N-P-A-A-S-S-A-G-G-I-L
+2 strand:  V-C-STOP-Y-H-V-P-R-I-T-Q-P-P-V-P-L-A-A-F
+3 strand:  Y-A-N-T-M-F-R-V-STOP-P-S-R-Q-F-R-W-R-H-F
-1 strand:   STOP-N-A-A-S-G-T-G-G-W-V-I-R-G-T-W-STOP-H-T
-2 strand:   K-M-P-P-A-E-L-A-A-G-L-Y-A-E-H-G-I-S-I
-3 strand:   K-C-R-Q-R-N-W-R-L-G-Y-T-R-N-M-V-L-A-Y

Open reading frames: Strands +1, -2, and -3

Ajvree (talk) 09:34, 6 September 2013 (PDT)

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