Difference between revisions of "Stephen Louie Week 2"
From LMU BioDB 2013
(Added corrections to +3) |
(Added corrections to the negative reading frames) |
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I-R-L-W-Y-K-A-H-I-G-S-A-V-K-A-T-A-V-K | I-R-L-W-Y-K-A-H-I-G-S-A-V-K-A-T-A-V-K | ||
| − | '''Y A N T | + | '''Y A N T M F R V Stop P S R Q F R W R H F''' |
====-1==== | ====-1==== | ||
I-L-R-R-S-P-STOP | I-L-R-R-S-P-STOP | ||
| + | |||
| + | '''Stop N A A S G T G G W V I R G T W Y Stop H T''' | ||
====-2==== | ====-2==== | ||
F-Y-G-G-R-L-D-R-R-P-N-M-R-L-V-P-Stop | F-Y-G-G-R-L-D-R-R-P-N-M-R-L-V-P-Stop | ||
| + | |||
| + | '''K M P P A E L A A G L Y A E H G I S I''' | ||
====-3==== | ====-3==== | ||
F-T-A-V-A-L-T-A-D-P-I-C-A-L-Y-H-N-V-M | F-T-A-V-A-L-T-A-D-P-I-C-A-L-Y-H-N-V-M | ||
| + | |||
| + | '''K C R Q R N W R L G Y T R N M V L A Y''' | ||
==Note== | ==Note== | ||
| Line 46: | Line 52: | ||
+2,+3,-3 are open reading frames | +2,+3,-3 are open reading frames | ||
| + | '''+1, -2 and -3 are open reading frames''' | ||
| + | |||
| + | ==Comments== | ||
| + | |||
| + | The reason for my mistakes on Reading Frames +1, +2, and +3 was difficult to discern initially. At first I was wondering whether it had to do with a mistake within the sequence. I realized that my process of translation was incorrect. When I was translating, I would convert the coding nucleotides into their complements, then look for the matching codon. In essence, I had the -1 reading frame of the complementary strand when I thought I was getting the +1 reading frame for the coding strand. | ||
==Other links== | ==Other links== | ||
Latest revision as of 23:46, 12 September 2013
Contents |
[edit] Genetic Code
[edit] Coding Strand
5’-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta-3’
[edit] Complementary Strand
3'-GCATACGATTATGGTACAAGGCGCATATTGGGTCGGCGGTCAAGGCGACCGCCGTAAAAT-5'
[edit] Possible Reading Frames
[edit] +1
A-Y-D-Y-G-T-R-R-I-L-G-R-R-S-R-R-P-P-Stop
R M L I P C S A Y N P A A S S A G G I L
[edit] +2
H-T-I-M-V-Q-G-A-Y-W-V-G-G-Q-G-D-R-R-K
V C Stop Y H V P R I T Q P P V P L A A F
[edit] +3
I-R-L-W-Y-K-A-H-I-G-S-A-V-K-A-T-A-V-K
Y A N T M F R V Stop P S R Q F R W R H F
[edit] -1
I-L-R-R-S-P-STOP
Stop N A A S G T G G W V I R G T W Y Stop H T
[edit] -2
F-Y-G-G-R-L-D-R-R-P-N-M-R-L-V-P-Stop
K M P P A E L A A G L Y A E H G I S I
[edit] -3
F-T-A-V-A-L-T-A-D-P-I-C-A-L-Y-H-N-V-M
K C R Q R N W R L G Y T R N M V L A Y
[edit] Note
+2,+3,-3 are open reading frames
+1, -2 and -3 are open reading frames
[edit] Comments
The reason for my mistakes on Reading Frames +1, +2, and +3 was difficult to discern initially. At first I was wondering whether it had to do with a mistake within the sequence. I realized that my process of translation was incorrect. When I was translating, I would convert the coding nucleotides into their complements, then look for the matching codon. In essence, I had the -1 reading frame of the complementary strand when I thought I was getting the +1 reading frame for the coding strand.
[edit] Other links
- Main Page
- My User Page
- Team Page
- Stephen Louie Project Notebook
- Week 1
- Class Journal 1
- Week 2
- Class Journal 2
- Stephen Louie Week 2
- Week 3
- Class Journal 3
- Stephen Louie Week 3
- Week 4
- Class Journal 4
- Stephen Louie Week 4
- Week 5
- Class Journal 5
- Stephen Louie Week 5
- Week 6
- Class Journal 6
- Stephen Louie Week 6
- Week 7
- Class Journal 7
- Stephen Louie Week 7
- Week 8
- Class Journal 8
- Stephen Louie Week 8
- Week 9
- Class Journal 9
- Stephen Louie Week 9
- Week 10
- Class Journal 10
- Stephen Louie Week 10
- Week 11
- Class Journal 11
- Stephen Louie Week 11
- Week 12
- Class Journal 12
- Stephen Louie Week 12
