Difference between revisions of "Stephen Louie Week 7"

Latest revision as of 06:28, 10 October 2013

[edit] Introduction to Microarrays

• 6b.

gene x: 1.0 (Black), 2.2 (Dim Red), 1.0 (Black), .15 (Medium Green)
gene y: 1.0 (Black), 4.5 (Medium Red), .95 (Black), .05 (Bright Green)
gene z: 1.0 (Black), 1.5 (Dim Red), 2.0 (Dim Red), 2.0 (Dim Red)

• 7.

Gene X and Gene Y were transcribed similarly.

• 9.

At the first point, most of the genes have not been acted upon.  Since they have neither been repressed or induced, the mRNA quantity is normal.  The red and green 1:1 ration is thus represented by yellow.

• 10.

TEF4 was repressed in this experiment.  When there is a lack of glucose, the cell has less fuel to convert to energy that is used to power activities.  Thus, the cell is forced to decrease its activity and repress the gene in order to conserve its limited supply of fuel.

• 11.

TCA genes are induced to provide an alternative form of energy to the cell when glucose is low.

• 12.

Regulatory regions are used to control gene expression.  Genes in common pathways can have shared promoter sequences or regulatory sequences so that they can all be controlled simultaneously. 

• 13.

You would see the color red.

• 14.

You can expect to see red spots.

• 15

Yes, a gene on a common pathway can be repressed by the overexpression or repression of a gene neighboring it due to an overflow of common transcription factors.

• 16

To be able to accurately tell if a gene is over-expressed or repressed, you should use a yellow color spot.  A change in color to red or green will indicate if the gene was truly over-expressed or repressed.

Slouie (talk) 22:59, 9 October 2013 (PDT)