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| − | Digital Notebook:
| + | [[Week_8| Week 8]] Individual Journal |
| − | 1) Downloaded original data (Merrell_Compiled_Raw_Data_Vibrio.xls) from [[http://www.openwetware.org/wiki/BIOL398-01/S10:Sample_Microarray_Analysis_Vibrio_cholerae]]
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| − | 2) Observed that the data collected had already been log transformed (there were negative numbers)
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| − | *Meant we could begin at the normalization step
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| − | 3) Created a new sheet in Excell, titled scaled_centered.
| + | |
| − | 4) In scaled_centered, inserted two new rows and calculated average and standard deviation for each replicate using the excell functions AVERAGE and STDEV.
| + | |
| − | 5) Created a new column for each of the samples, relabeling them with a _sc (for scaled centered) after the name. Filled these columns with the scaled centered values calculated by taking the raw data minus the average for the sample (row 2) divided by the standard deviation (row 3).
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