Difference between revisions of "Ajvree Week 2"
From LMU BioDB 2013
(created complementary DNA strand) |
(+1,2,3 reading frames) |
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* Write out the complementary strand of DNA below the strand shown and be sure to label the 5’ and 3’ ends of the complementary strand. | * Write out the complementary strand of DNA below the strand shown and be sure to label the 5’ and 3’ ends of the complementary strand. | ||
| − | 5’- | + | 5’-cgt-atg-cta-ata-cca-tgt-tcc-gcg-tat-aac-cca-gcc-gcc-agt-tcc-gct-ggc-ggc-att-tta-3’ |
| − | 3'- | + | 3'-gca-tac-gat-tat-ggt-aca-agg-cgc-ata-ttg-ggt-cgg-cgg-tca-agg-cga-ccg-ccg-taa-aat-5' |
* There are six possible reading frames in double-stranded DNA. Using the [http://en.wikipedia.org/wiki/Genetic_code#RNA_codon_table genetic code], translate all possible reading frames of this DNA sequence, keeping in mind the following rules. | * There are six possible reading frames in double-stranded DNA. Using the [http://en.wikipedia.org/wiki/Genetic_code#RNA_codon_table genetic code], translate all possible reading frames of this DNA sequence, keeping in mind the following rules. | ||
| + | |||
| + | +1 strand: R-M-L-I-P-C-S-A-Y-N-P-A-A-S-S-A-G-G-I-L | ||
| + | +2 strand: V-C-STOP | ||
| + | +3 strand: Y-A-N-T-M-F-R-V-STOP | ||
| + | |||
** In RNA, the T becomes a U, so everywhere you see a T in the sequence, read it as a U. | ** In RNA, the T becomes a U, so everywhere you see a T in the sequence, read it as a U. | ||
** The genetic code is read in the 5’ to 3’ direction. | ** The genetic code is read in the 5’ to 3’ direction. | ||
Revision as of 16:03, 6 September 2013
- Write out the complementary strand of DNA below the strand shown and be sure to label the 5’ and 3’ ends of the complementary strand.
5’-cgt-atg-cta-ata-cca-tgt-tcc-gcg-tat-aac-cca-gcc-gcc-agt-tcc-gct-ggc-ggc-att-tta-3’
3'-gca-tac-gat-tat-ggt-aca-agg-cgc-ata-ttg-ggt-cgg-cgg-tca-agg-cga-ccg-ccg-taa-aat-5'
- There are six possible reading frames in double-stranded DNA. Using the genetic code, translate all possible reading frames of this DNA sequence, keeping in mind the following rules.
+1 strand: R-M-L-I-P-C-S-A-Y-N-P-A-A-S-S-A-G-G-I-L +2 strand: V-C-STOP +3 strand: Y-A-N-T-M-F-R-V-STOP
- In RNA, the T becomes a U, so everywhere you see a T in the sequence, read it as a U.
- The genetic code is read in the 5’ to 3’ direction.
- Use the single-letter abbreviations for the amino acids because that is what is commonly used by computer programs.
- Wiki Hint: if you start your line with a single space character, it will appear in the grey box surrounded by the dashed line as shown above.
- Which of the reading frames (if any) of the reading frames you translated is an open reading frame, i.e., does not contain a stop codon?
- By convention, the top strand frames are called +1, +2, +3, reading 5' to 3' and the bottom strand frames are called -1, -2, -3, reading 5' to 3'.
