Difference between revisions of "Stephen Louie Week 2"

Revision as of 06:57, 12 September 2013

Genetic Code

Coding Strand

5’-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta-3’

Complementary Strand

3'-GCATACGATTATGGTACAAGGCGCATATTGGGTCGGCGGTCAAGGCGACCGCCGTAAAAT-5'

Possible Reading Frames

+1

A-Y-D-Y-G-T-R-R-I-L-G-R-R-S-R-R-P-P-Stop

R M L I P C S A Y N P A A S S A G G I L

+2

H-T-I-M-V-Q-G-A-Y-W-V-G-G-Q-G-D-R-R-K

V C Stop Y H V P R I T Q P P V P L A A F

+3

I-R-L-W-Y-K-A-H-I-G-S-A-V-K-A-T-A-V-K

Y A N T M F R V Stop P S R Q F R W R H F

-1

I-L-R-R-S-P-STOP

I L R R S P Stop P P T Q Y A P C T I I V C

-2

F-Y-G-G-R-L-D-R-R-P-N-M-R-L-V-P-Stop

F Y G G R L D R R P N M R L V P Stop S Y

-3

F-T-A-V-A-L-T-A-D-P-I-C-A-L-Y-H-N-V-M

F T A V A L T A D P I C A L Y H N R M

Note

+2,+3,-3 are open reading frames

+1 and -3 are open reading frames

Comments

The reason for my mistakes on Reading Frames +1, +2, and +3 was difficult to discern initially. At first I was wondering whether it had to do with a mistake within the sequence. I realized that my process of translation was incorrect. When I was translating, I would convert the coding nucleotides into their complements, then look for the matching codon. In essence, I had the -1 reading frame of the complementary strand when I thought I was getting the +1 reading frame for the coding strand.

Other links

• Main Page
• My User Page
• Team Page
• Stephen Louie Project Notebook
• Week 1
• Class Journal 1
• Week 2
• Class Journal 2
• Stephen Louie Week 2
• Week 3
• Class Journal 3
• Stephen Louie Week 3
• Week 4
• Class Journal 4
• Stephen Louie Week 4
• Week 5
• Class Journal 5
• Stephen Louie Week 5
• Week 6
• Class Journal 6
• Stephen Louie Week 6
• Week 7
• Class Journal 7
• Stephen Louie Week 7
• Week 8
• Class Journal 8
• Stephen Louie Week 8
• Week 9
• Class Journal 9
• Stephen Louie Week 9
• Week 10
• Class Journal 10
• Stephen Louie Week 10
• Week 11
• Class Journal 11
• Stephen Louie Week 11
• Week 12
• Class Journal 12
• Stephen Louie Week 12