Difference between revisions of "Laurmagee: Week 4"
From LMU BioDB 2013
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| − | + | ===Transcription and Translation “Taken to the Next Level”=== | |
| − | + | ==-10 box== | |
| − | + | #cat [file] | sed "s/[ct]at[at]at/ -10 box/g" | |
| + | #cat [file] | sed "s/[ct]at[at]at/ <minus10box>&<\/minus10box> /g" | ||
| − | + | ==Added -35 box== | |
| − | + | #cat [file] | sed "s/tt[gt]ac[at]/ -35 box/g" | |
| − | + | #cat [file] | sed "s/tt[gt]ac[at]/ <minus35box>&<\/minus35box> /g" | |
| + | #cat [file] | sed "s/[ct]at[at]at/ <minus10box>&<\/minus10box> /g" | sed "s/................. <minus10/ <\/minus35box> &/g" | ||
| + | #cat [file] | sed "s/[ct]at[at]at/ <minus10box>&<\/minus10box> /g" | sed -r "s/.{17} <minus10/ <\/minus35box> &/g" | ||
| + | #cat [file] | sed "s/[ct]at[at]at/ <minus10box>&<\/minus10box> /g" | sed -r "s/.{17} <minus10/ <\/minus35box> &/g" | sed "s/"s/tt[gt]ac[at]<\/minus35box>/ <minus35box>&/g" | ||
| − | + | ==Added Ribosome Binding Site== | |
| − | + | #cat [file] | sed "s/gag/ <rbs>&<\/rbs> /g" | |
| − | + | #cat [file] | sed "s/gag/ <rbs>&<\/rbs> /g" | sed "s/atg/start/g" | |
| − | + | ||
| − | + | ==Added Start Codon= | |
| − | + | #cat [file] | sed "s/gag/ <rbs>&<\/rbs> /g" | sed "s/<\/rbs>/&\n/g" | sed "2s/atg/ <start_codon>&<\/start_codon> /1" | |
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[[Category: Journal Entry]] | [[Category: Journal Entry]] | ||
Revision as of 08:55, 19 September 2013
Contents |
Transcription and Translation “Taken to the Next Level”
-10 box
- cat [file] | sed "s/[ct]at[at]at/ -10 box/g"
- cat [file] | sed "s/[ct]at[at]at/ <minus10box>&<\/minus10box> /g"
Added -35 box
- cat [file] | sed "s/tt[gt]ac[at]/ -35 box/g"
- cat [file] | sed "s/tt[gt]ac[at]/ <minus35box>&<\/minus35box> /g"
- cat [file] | sed "s/[ct]at[at]at/ <minus10box>&<\/minus10box> /g" | sed "s/................. <minus10/ <\/minus35box> &/g"
- cat [file] | sed "s/[ct]at[at]at/ <minus10box>&<\/minus10box> /g" | sed -r "s/.{17} <minus10/ <\/minus35box> &/g"
- cat [file] | sed "s/[ct]at[at]at/ <minus10box>&<\/minus10box> /g" | sed -r "s/.{17} <minus10/ <\/minus35box> &/g" | sed "s/"s/tt[gt]ac[at]<\/minus35box>/ <minus35box>&/g"
Added Ribosome Binding Site
- cat [file] | sed "s/gag/ <rbs>&<\/rbs> /g"
- cat [file] | sed "s/gag/ <rbs>&<\/rbs> /g" | sed "s/atg/start/g"
=Added Start Codon
- cat [file] | sed "s/gag/ <rbs>&<\/rbs> /g" | sed "s/<\/rbs>/&\n/g" | sed "2s/atg/ <start_codon>&<\/start_codon> /1"
