Difference between revisions of "Kmeilak Week 4"
From LMU BioDB 2013
| Line 3: | Line 3: | ||
===Transcription and Translation “Taken to the Next Level”=== | ===Transcription and Translation “Taken to the Next Level”=== | ||
| − | 1. | + | ====1. code==== |
cat infA-E.coli-K12.txt | sed "s/cat[at]at/ <minus10box> & <\/minus10box> /g" | sed -r "s/.{17} <minus10box>/ <\/minus35box> &/g" | sed -r "s/.{6} <\/minus35box>/ <minus35box> &/g" | sed -r "s/ <minus10box> .{6} <\/minus10box> .{5}/& <tss> /g" | sed "s/ <tss> ./& <\/tss> /g" | sed "s/gagg/ <rbs> & <\/rbs>\n/g" | sed "2s/atg/ <start_codon> & <\/start_codon> /1" | sed -r "2s/<\/start_codon> ...*t[ag][ag]/& <\/stop_codon> /g" | sed "2s/... <\/stop_codon>/ <stop_codon> &/g" | sed "2s/aaaaggt/ <terminator> &/g" | sed "2s/tttt..../& <\/terminator> /2" | cat infA-E.coli-K12.txt | sed "s/cat[at]at/ <minus10box> & <\/minus10box> /g" | sed -r "s/.{17} <minus10box>/ <\/minus35box> &/g" | sed -r "s/.{6} <\/minus35box>/ <minus35box> &/g" | sed -r "s/ <minus10box> .{6} <\/minus10box> .{5}/& <tss> /g" | sed "s/ <tss> ./& <\/tss> /g" | sed "s/gagg/ <rbs> & <\/rbs>\n/g" | sed "2s/atg/ <start_codon> & <\/start_codon> /1" | sed -r "2s/<\/start_codon> ...*t[ag][ag]/& <\/stop_codon> /g" | sed "2s/... <\/stop_codon>/ <stop_codon> &/g" | sed "2s/aaaaggt/ <terminator> &/g" | sed "2s/tttt..../& <\/terminator> /2" | ||
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aaaaggtcggtttaaccggcctttttatt | aaaaggtcggtttaaccggcctttttatt | ||
| − | 2. mRNA sequence | + | |
| + | ====2. mRNA sequence==== | ||
gccaaguuuaaugccaucacuauggggucuccuaaucuaccgguuucuucuguuauaacuuuacguuccauggcaagaacuuugcaacggauuaugguacaaggcgcaucucaaucuuuugccagugcaccaaugacguguguagaggccauuuuacgcguuuuugauguaggcguaggacugcccgcuguuucacugacaacuugacuggggcaugcuggacucguuuccggcguaacagaaggcaucagcgacuaacaaaauggcggacuacccgcuucucuuucuugcucauuuuccagccaaauuggccggaaaaauaa | gccaaguuuaaugccaucacuauggggucuccuaaucuaccgguuucuucuguuauaacuuuacguuccauggcaagaacuuugcaacggauuaugguacaaggcgcaucucaaucuuuugccagugcaccaaugacguguguagaggccauuuuacgcguuuuugauguaggcguaggacugcccgcuguuucacugacaacuugacuggggcaugcuggacucguuuccggcguaacagaaggcaucagcgacuaacaaaauggcggacuacccgcuucucuuucuugcucauuuuccagccaaauuggccggaaaaauaa | ||
| − | 3. Amino Acid sequence | + | |
| + | ====3. Amino Acid sequence==== | ||
M G S P N L P V S S V I T L R S M A R T L Q R I M V Q G A S Q S F A S A P M T C V E A I L R V F D V G V G L P A V S L T T | M G S P N L P V S S V I T L R S M A R T L Q R I M V Q G A S Q S F A S A P M T C V E A I L R V F D V G V G L P A V S L T T | ||
Latest revision as of 04:40, 20 September 2013
Contents |
[edit] Week 4
[edit] Transcription and Translation “Taken to the Next Level”
[edit] 1. code
cat infA-E.coli-K12.txt | sed "s/cat[at]at/ <minus10box> & <\/minus10box> /g" | sed -r "s/.{17} <minus10box>/ <\/minus35box> &/g" | sed -r "s/.{6} <\/minus35box>/ <minus35box> &/g" | sed -r "s/ <minus10box> .{6} <\/minus10box> .{5}/& <tss> /g" | sed "s/ <tss> ./& <\/tss> /g" | sed "s/gagg/ <rbs> & <\/rbs>\n/g" | sed "2s/atg/ <start_codon> & <\/start_codon> /1" | sed -r "2s/<\/start_codon> ...*t[ag][ag]/& <\/stop_codon> /g" | sed "2s/... <\/stop_codon>/ <stop_codon> &/g" | sed "2s/aaaaggt/ <terminator> &/g" | sed "2s/tttt..../& <\/terminator> /2"
- sequence of -35 box
tttact
- sequence of -10 box
cattat
- transcription start site base pair
c
- sequence of ribosome binding site
gagg
- start codon
atg
- stop codon
tga
- terminator sequence
aaaaggtcggtttaaccggcctttttatt
[edit] 2. mRNA sequence
gccaaguuuaaugccaucacuauggggucuccuaaucuaccgguuucuucuguuauaacuuuacguuccauggcaagaacuuugcaacggauuaugguacaaggcgcaucucaaucuuuugccagugcaccaaugacguguguagaggccauuuuacgcguuuuugauguaggcguaggacugcccgcuguuucacugacaacuugacuggggcaugcuggacucguuuccggcguaacagaaggcaucagcgacuaacaaaauggcggacuacccgcuucucuuucuugcucauuuuccagccaaauuggccggaaaaauaa
[edit] 3. Amino Acid sequence
M G S P N L P V S S V I T L R S M A R T L Q R I M V Q G A S Q S F A S A P M T C V E A I L R V F D V G V G L P A V S L T T
- Main Page
- Kevin's User Page
- Week 1
- Week 2
- Week 3
- Week 4
- Week 5
- Week 6
- Week 7
- Week 8
- Week 9
- Week 10
- Week 11
- Week 12
- Week 13
- Week 14
- Week 15
- Class Journal Week 1
- Class Journal Week 2
- Class Journal Week 3
- Class Journal Week 4
- Class Journal Week 5
- Class Journal Week 6
- Class Journal Week 7
- Class Journal Week 8
- Class Journal Week 9
- Class Journal Week 10
- Class Journal Week 11
- Class Journal Week 12
- Class Journal Week 13
- Class Journal Week 14
- Class Journal Week 15
- Kmeilak Week 2
- Kmeilak Week 3
- Kmeilak Week 4
- Kmeilak Week 5
- Kmeilak Week 6
- Kmeilak Week 7
- Kmeilak Week 8
- Kmeilak Week 9
- Kmeilak Week 10
- Kmeilak Week 11
- Kmeilak Week 12
- Kmeilak Week 13
- Kmeilak Week 14
- Kmeilak Week 15
