Difference between revisions of "Ksherbina Week 5"

Revision as of 01:41, 27 September 2013

Steps to Look Up Human EGFR Protein in the UniProt Database

1. Go to the UniProt Database.
2. Type the UniProt ID P00533 into the Query field in the top middle of the page.
3. Click the Search button.
4. Locate the navigation toolbar, which contains links to different sections in a light blue box with rounded corners that spans the page horizontally.
5. Click on the link "Entry information" to jump to that section.
   • This section contains information regarding Entry name (for quick identification), Accession Number (unique identifier for the protein), Entry history, Entry status, and Annotation program (these headers appear in a vertical column to the left).
6. In the row of this section containing accession number information, click on the gray icon with two arrows pointing in opposite directions to expand the list of secondary accession numbers for the protein.
7. Scroll back to the top of the page.
8. Type the secondary accession number Q14225 for the protein into the Query filed in the middle of the page.
9. Click the search button.
   • You should be redirected to the page that you were already browsing through.
10. Click on the link "Names" in the navigation tool bar. This should take you to a section called "Names and origin".
    • This section should contain information regarding Protein names, Gene names, Organism, Taxonomic identifier, and Taxonomic lineage. (these headers appear in a vertical column to the left).
11. Click on the Enzyme Commission number, which is listed after "EC=" in the row designed "Protein names".
    • This will take you to a page in the database ExPASy which gives information regarding what is the biochemical function of human EGFR.
12. Return to the previous page in the UniProt database.

Summary of Information on the Human EGFR Protein

<--! It is a type of tyrosine kinase. -->