Difference between revisions of "Individual hw week 8"
| Line 34: | Line 34: | ||
When I compared my list to stephen's it was apparent that they were different. I believe that this difference arises from the different versions of the data that we used. Mine was from 2009 and his was from 2010. | When I compared my list to stephen's it was apparent that they were different. I believe that this difference arises from the different versions of the data that we used. Mine was from 2009 and his was from 2010. | ||
| + | |||
| + | ===GO Search=== | ||
| + | |||
| + | VC0647- 3'-5' exoribonuclease activity | ||
| + | VCA0583- outer membrane-bounded periplasmic space | ||
| + | |||
| + | I looked up the genes associated with VC0647 and chose the gene DSBA_VIBCH. This gene encodes for Thiol:Disulfate interchange protein. This gene's expression increased during this experiment. This protein is responsible for the formation of disulfide bonds.This protein is useful for its involvement in the secretion of virulence factors. | ||
| + | |||
[[Media:Increased_MPM-Criterion1-GO.txt]]<br> | [[Media:Increased_MPM-Criterion1-GO.txt]]<br> | ||
Revision as of 17:04, 17 October 2013
part 1 text file
Media:Merrell Compiled Raw Data Vibrio MPM.txt
Part 1 excel file
Media:Merrell_Compiled_Raw_Data_Vibrio_MPM.xls
948 results
During this lab I mapped the increased expression on the 2009 gene database. For some reason in the beginning of this assignment my .txt file did not want to load so I used my partner's
the top 10 gene ontology terms were
1.Protein folding
2.Chorismate metabolic process
3.aromatic amino acid biosynthesis process
4. cytoplasm
5.protein-N(PI)-phosphohistidine-sugar phosphotransferase activity
6.solute:hydrogen symporter activity
7.sugar:hydrogen symporter activity
8.cation:sugar symporter activity
9.phosphoenolpyruvate-dependent sugar phosphotransferase system
10.zinc ion binding
When I compared my list to stephen's it was apparent that they were different. I believe that this difference arises from the different versions of the data that we used. Mine was from 2009 and his was from 2010.
GO Search
VC0647- 3'-5' exoribonuclease activity VCA0583- outer membrane-bounded periplasmic space
I looked up the genes associated with VC0647 and chose the gene DSBA_VIBCH. This gene encodes for Thiol:Disulfate interchange protein. This gene's expression increased during this experiment. This protein is responsible for the formation of disulfide bonds.This protein is useful for its involvement in the secretion of virulence factors.
Media:Increased_MPM-Criterion1-GO.txt
Media:Merrell_Compiled_Raw_Data_Vibrio_SL_10102013.gex
Media:MAPPFinder_GO.txt_excel_file_MPM.xls
Media:Merrell_Compiled_Raw_Data_Vibrio_SL_10102013.EX.txt
Media:Merrell_Compiled_Raw_Data_Vibrio_SL_10102013.gmf
