Difference between revisions of "Ajvree Week 10"
From LMU BioDB 2013
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*Searched article for location of microarray data, located here: '''http://www.ncbi.nlm.nih.gov/geo''' under | *Searched article for location of microarray data, located here: '''http://www.ncbi.nlm.nih.gov/geo''' under | ||
accession number '''GSE34751''' | accession number '''GSE34751''' | ||
| + | *In this experiment, cell cultures were exposed to 4 different translation inhibitors to determine the effect on the transcript activity. The control used was a 1% DMSO culture that contained no antibiotics. There were 3 replicates done of the microarray analysis, and Northern blot analysis was also used. | ||
*'''PDF:''' [[Media:Geneexpressionarticle1.pdf|S. pneumoniae Article 1]] | *'''PDF:''' [[Media:Geneexpressionarticle1.pdf|S. pneumoniae Article 1]] | ||
*Full Citation: <br> | *Full Citation: <br> | ||
Revision as of 23:28, 1 November 2013
10/29/13 Lecture Notes
- Library homepage>Libguides>link to BIO 367>databases
- Open access: physics(peer sharing) and life sciences (availability of granted research)
- PMC Journal: information designated by govt to be available to all
- Now authors pay journal to have information published, makes open access controversial
- http://libguides.lmu.edu/BIOL367
- Google scholar ranks articles, dates will be all over the place, etc.
- MeSH terms: medical subheading
- information needed: a reference genome for species/main reference strand, usually the earliest date
Search Results:
Article 1:
- Searched Web of Science for transcriptional profiling Streptococcus pneumoniae under topic, got 38 results.
- Found article titled : Gene expression profile of early in vitro biofilms of Streptococcus pneumoniae (Mukesh Kumar Yadav et al., 2012)
- Used Google Scholar to search for full article
- Article PDF involves comparing microarray data of S. pneumoniae biofilm to planktonic cells
- Searched article for location of microarray data, located here: http://www.ncbi.nlm.nih.gov/geo under
accession number GSE34751
- In this experiment, cell cultures were exposed to 4 different translation inhibitors to determine the effect on the transcript activity. The control used was a 1% DMSO culture that contained no antibiotics. There were 3 replicates done of the microarray analysis, and Northern blot analysis was also used.
- PDF: S. pneumoniae Article 1
- Full Citation:
Yadav, M. K., Kwon, S. K., Cho, C. G., Park, S.-W., Chae, S.-W. and Song, J.-J. (2012), Gene expression profile of early in vitro biofilms of Streptococcus pneumoniae. Microbiology and Immunology, 56: 621–629.
- Based on this report and other titles listed while searching, a lot of research regarding vaccines against the pathogenic strain is popular, in addition to research on how to prevent the bacteria from growing.
Article 2:
- Found second article using references from first article, looked for key words in title about transcription activity/microarray data
- link to article: [1]
- Full Citation:
Ng, WL, Kazmierczak, KM Robertson, GT, et al., 2003. Transcriptional regulation and signature patterns revealed by microarray analyses of Streptococcus pneumoniae R6 challenged with sublethal concentrations of translation inhibitors. Journal of Bacteriology. Volume 185, Issue 1, Pages 359-370.
- This experiment looked at what happened to transcript activity after being exposed to multiple lethal transcription inhibitors.
