Difference between revisions of "Kevinmcgee Week 13"

From LMU BioDB 2013
Jump to: navigation, search
(Added info to journal)
Line 2: Line 2:
 
#Finished the formatting by flipping the dye swap chips negative
 
#Finished the formatting by flipping the dye swap chips negative
 
##Created a column next to dye swap chips and did the formula:  
 
##Created a column next to dye swap chips and did the formula:  
=-1*(dye swap chip column)
+
##* =-1*(dye swap chip column)
 +
#made a new sheet
 +
##added all data from old sheet except only added the flipped dye swaps
 
#looked for background information in the array paper
 
#looked for background information in the array paper
 
##L. infantum MHOM/MA/67/ITMAP-263 and L. major LV39 MRHO/SU/59/P strains used in this study
 
##L. infantum MHOM/MA/67/ITMAP-263 and L. major LV39 MRHO/SU/59/P strains used in this study
Line 8: Line 10:
 
##*The series accession number for our manuscript is GSE10407.
 
##*The series accession number for our manuscript is GSE10407.
 
##Each chip compares promastigote vs. amastigote with different replicates
 
##Each chip compares promastigote vs. amastigote with different replicates
 +
##Following data files found
 +
##* GSM291421 L. infantum MOHM/MA/67/ITMAP-263 promastigote vs intracellular amastigote_replicate 1
 +
##* GSM291422 L. infantum MOHM/MA/67/ITMAP-263 promastigote vs intracellular amastigote_replicate 2
 +
##* GSM291423 L. infantum MOHM/MA/67/ITMAP-263 promastigote vs intracellular amastigote_replicate 3
 +
##* GSM291424 L. infantum MOHM/MA/67/ITMAP-263 promastigote vs intracellular amastigote_replicate 4
 +
##* GSM291425 L. infantum MOHM/MA/67/ITMAP-263 promastigote vs intracellular amastigote_replicate 5
 +
##* GSM291426 L. infantum MOHM/MA/67/ITMAP-263 promastigote vs intracellular amastigote_replicate 6
 +
##* GSM291427 L.major LV39 promastigote vs intracellular amastigote_replicate 1
 +
##* GSM291428 L.major LV39 promastigote vs intracellular amastigote_replicate 2
 +
##* GSM291429 L.major LV39 promastigote vs intracellular amastigote_replicate 3
 +
##* GSM291430 L.major LV39 promastigote vs intracellular amastigote_replicate 4

Revision as of 18:38, 19 November 2013

  1. Opened L.infantumCompliedRawData(A).txt
  2. Finished the formatting by flipping the dye swap chips negative
    1. Created a column next to dye swap chips and did the formula:
      • =-1*(dye swap chip column)
  3. made a new sheet
    1. added all data from old sheet except only added the flipped dye swaps
  4. looked for background information in the array paper
    1. L. infantum MHOM/MA/67/ITMAP-263 and L. major LV39 MRHO/SU/59/P strains used in this study
    2. All microarray data will be freely available on the Geo NCBI database in the MIAME format
      • The series accession number for our manuscript is GSE10407.
    3. Each chip compares promastigote vs. amastigote with different replicates
    4. Following data files found
      • GSM291421 L. infantum MOHM/MA/67/ITMAP-263 promastigote vs intracellular amastigote_replicate 1
      • GSM291422 L. infantum MOHM/MA/67/ITMAP-263 promastigote vs intracellular amastigote_replicate 2
      • GSM291423 L. infantum MOHM/MA/67/ITMAP-263 promastigote vs intracellular amastigote_replicate 3
      • GSM291424 L. infantum MOHM/MA/67/ITMAP-263 promastigote vs intracellular amastigote_replicate 4
      • GSM291425 L. infantum MOHM/MA/67/ITMAP-263 promastigote vs intracellular amastigote_replicate 5
      • GSM291426 L. infantum MOHM/MA/67/ITMAP-263 promastigote vs intracellular amastigote_replicate 6
      • GSM291427 L.major LV39 promastigote vs intracellular amastigote_replicate 1
      • GSM291428 L.major LV39 promastigote vs intracellular amastigote_replicate 2
      • GSM291429 L.major LV39 promastigote vs intracellular amastigote_replicate 3
      • GSM291430 L.major LV39 promastigote vs intracellular amastigote_replicate 4
Personal tools
Namespaces

Variants
Actions
Navigation
Toolbox