Difference between revisions of "Kevinmcgee Week 13"

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(Added info to journal)
(Added pic)
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##Each chip compares promastigote vs. amastigote with different replicates
 
##Each chip compares promastigote vs. amastigote with different replicates
 
##Following data files found
 
##Following data files found
##* GSM291421 L. infantum MOHM/MA/67/ITMAP-263 promastigote vs intracellular amastigote_replicate 1
+
[[File:LmjSampleInfo.PNG]]
##* GSM291422 L. infantum MOHM/MA/67/ITMAP-263 promastigote vs intracellular amastigote_replicate 2
+
##* GSM291423 L. infantum MOHM/MA/67/ITMAP-263 promastigote vs intracellular amastigote_replicate 3
+
##* GSM291424 L. infantum MOHM/MA/67/ITMAP-263 promastigote vs intracellular amastigote_replicate 4
+
##* GSM291425 L. infantum MOHM/MA/67/ITMAP-263 promastigote vs intracellular amastigote_replicate 5
+
##* GSM291426 L. infantum MOHM/MA/67/ITMAP-263 promastigote vs intracellular amastigote_replicate 6
+
##* GSM291427 L.major LV39 promastigote vs intracellular amastigote_replicate 1
+
##* GSM291428 L.major LV39 promastigote vs intracellular amastigote_replicate 2
+
##* GSM291429 L.major LV39 promastigote vs intracellular amastigote_replicate 3
+
##* GSM291430 L.major LV39 promastigote vs intracellular amastigote_replicate 4
+
 
#Finished naming sheet with helpful names to know what is what on the sheet
 
#Finished naming sheet with helpful names to know what is what on the sheet
 
#Ready for statistical analysis
 
#Ready for statistical analysis

Revision as of 18:51, 19 November 2013

  1. Opened L.infantumCompliedRawData(A).txt
  2. Finished the formatting by flipping the dye swap chips negative
    1. Created a column next to dye swap chips and did the formula:
      • =-1*(dye swap chip column)
  3. made a new sheet
    1. added all data from old sheet except only added the flipped dye swaps
  4. looked for background information in the array paper
    1. L. infantum MHOM/MA/67/ITMAP-263 and L. major LV39 MRHO/SU/59/P strains used in this study
    2. All microarray data will be freely available on the Geo NCBI database in the MIAME format
      • The series accession number for our manuscript is GSE10407.
    3. Each chip compares promastigote vs. amastigote with different replicates
    4. Following data files found

LmjSampleInfo.PNG

  1. Finished naming sheet with helpful names to know what is what on the sheet
  2. Ready for statistical analysis
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