Difference between revisions of "Vkuehn Week 13"
From LMU BioDB 2013
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:*data from several independent L. infantum and L. major DNA microarray experiments were compiled and compared | :*data from several independent L. infantum and L. major DNA microarray experiments were compiled and compared | ||
:*compared each gene according to the normalized log2 ratio of the Alexa 647/Alexa 555 signal intensities (amastigotes/promastigotes) and to the signal mean intensity of each spot | :*compared each gene according to the normalized log2 ratio of the Alexa 647/Alexa 555 signal intensities (amastigotes/promastigotes) and to the signal mean intensity of each spot | ||
| + | :*Transcriptional analyses of L. infantum promastigote compared to L. infantum intracellular amastigote, and L. major promastigote compared to L. major intracellular amastigote | ||
| + | The full-genome DNA microarray includes one 70mer-oligonucleotide probe for each gene of L. infantum and for each gene of L.major LV39 | ||
:*Lmj Sample Info: The replicate numbers and sample IDs correspond to our txt files. (22, 24, 25, 28, 30 were dye swaps) | :*Lmj Sample Info: The replicate numbers and sample IDs correspond to our txt files. (22, 24, 25, 28, 30 were dye swaps) | ||
[[File:LmjSampleInfo.PNG]] | [[File:LmjSampleInfo.PNG]] | ||
:You need to record a narrative that describes all of the methods that you are using in enough detail so that the instructors or your other team members can follow and reproduce your work. | :You need to record a narrative that describes all of the methods that you are using in enough detail so that the instructors or your other team members can follow and reproduce your work. | ||
Include links to artifacts you produce (files, images, testing reports, code, etc.) | Include links to artifacts you produce (files, images, testing reports, code, etc.) | ||
Revision as of 18:57, 19 November 2013
Week 13 Individual Lab Notebook
From this week on, your Individual Journal Assignment will be to complete your electronic laboratory notebook that records your work on your team's final project.
- Understanding the array data:
- data from several independent L. infantum and L. major DNA microarray experiments were compiled and compared
- compared each gene according to the normalized log2 ratio of the Alexa 647/Alexa 555 signal intensities (amastigotes/promastigotes) and to the signal mean intensity of each spot
- Transcriptional analyses of L. infantum promastigote compared to L. infantum intracellular amastigote, and L. major promastigote compared to L. major intracellular amastigote
The full-genome DNA microarray includes one 70mer-oligonucleotide probe for each gene of L. infantum and for each gene of L.major LV39
- Lmj Sample Info: The replicate numbers and sample IDs correspond to our txt files. (22, 24, 25, 28, 30 were dye swaps)
- You need to record a narrative that describes all of the methods that you are using in enough detail so that the instructors or your other team members can follow and reproduce your work.
Include links to artifacts you produce (files, images, testing reports, code, etc.)
