Difference between revisions of "Miles Malefyt deliverables"
From LMU BioDB 2013
(→week 15) |
(→week 15) |
||
| Line 37: | Line 37: | ||
[[Media:MAPPFinder_results_T15-Criterion_Increased-GO.txt|Increased expression at T15]] | [[Media:MAPPFinder_results_T15-Criterion_Increased-GO.txt|Increased expression at T15]] | ||
| + | |||
[[Media:MAPPFinder_results_T15-Criterion_Decreased-GO.txt|Decreased expression at T15]] | [[Media:MAPPFinder_results_T15-Criterion_Decreased-GO.txt|Decreased expression at T15]] | ||
| + | |||
| + | [[Media:T30_increased-Criterion0-GO.txt|Increased expression at t30 300mm NaCl]] | ||
| + | |||
| + | [[Media:T30_MAPP_results-Criterion_Decreased-GO.txt|Decreased expression at t30 300mm NaCl]] | ||
| + | |||
[[Media:MAPPFinder_t60-Criterion_Increased-GO.txt|Increased expression at t60]] | [[Media:MAPPFinder_t60-Criterion_Increased-GO.txt|Increased expression at t60]] | ||
| + | |||
[[Media:MAPPFinder_t60-Criterion_Decreasaed-GO.txt|Decreased expression at t60]] | [[Media:MAPPFinder_t60-Criterion_Decreasaed-GO.txt|Decreased expression at t60]] | ||
| + | |||
| + | [[Media:300N_T240_MAPPFINDER-Criterion_Increased-GO.txt|Increased expression t240 300mmNaCl]] | ||
| + | |||
| + | [[Media:300N_T240_MAPPFINDER-Criterion_Decreased-GO.txt|decreased expression at t240 300mmNaCl]] | ||
*analyzed the Gene Ontology report | *analyzed the Gene Ontology report | ||
**found a specific metabolic pathway that was pertinent to the treatment of NaCl | **found a specific metabolic pathway that was pertinent to the treatment of NaCl | ||
Latest revision as of 23:32, 13 December 2013
Contents |
[edit] Statement of work
[edit] Week 12
- Read the paper on the salinity and sucrose stress on gene expression
- Sorted the raw data into an XML file
- started to compile the raw data
- downloaded all raw data and sorted through the information needed
- used the cys5 and cys3 fold change as well as all the IDs
- Uploaded 300 NaCl compiled data set
[edit] Week 13
- I continued to sort the raw data and began to process the data in an xls file
- this was a very repetitive part because it involved a lot of replications for each time set
- finished my compiled raw data and processed raw data, as well as the data ready for GenMAPP
- Made individual LOG fold change ratios for each time point replicate then averaged all of the LOG fold change ratios for each of the time points
- preformed a Tstat test
- Preformed a Pvalue test
- added a row of N next to the gene ID name in the forGenMAPP tab
- uploaded Processed Data
- NOTE:the GenMAPP version of the tab is labeled Complete Processed data_MPM and not forGenMAPP
[edit] week 15
- I worked on some of the mistakes that I had made in my prior data sets
- removed AVG_LOGFC_ALL row
- added individual Pvalues and TSTAT for each individual replicate of the experiment instead of one Tstat and P value for the whole experiment
- sanity check concluded the number of genes significantly changed at each time point
- T15- 5520
- T30- 7484
- T60- 6711
- T240- 5901
- Removed all of the #DIV/0! from the data that was transferred over to the GenMapp data
- uploaded XLS Version and TXT version, USE THIS
- had to change names of the columns in order to correctly upload to GenMAPP
- system code column was renamed
- Gene ID column was renamed to ID on the Programmers computer to resolve some issues
- it appears that there is something wrong with the actual gene IDs that is not compatible with GenMAPP
- ran the first integration of the data and came up with 5535 errors which is roughly half of the overall genes we loaded.
- loaded genMAPP and Mappfinder results
Increased expression at t30 300mm NaCl
Decreased expression at t30 300mm NaCl
Increased expression t240 300mmNaCl
decreased expression at t240 300mmNaCl
- analyzed the Gene Ontology report
- found a specific metabolic pathway that was pertinent to the treatment of NaCl
