Difference between revisions of "Genetic Code Assignment"

Revision as of 04:40, 6 September 2013

Write out the complementary strand of DNA below the strand shown and be sure to label the 5’ and 3’ ends of the complementary strand.

5’-cgt atg cta   ata  cca  tgt tcc  gcg tat   aac cca   gcc gcc agt  tcc  gct ggc ggc att  tta-3’
3'-cga uac gau uau ggu aca agg cgc aua uug ggu cgg cgg uca agg cga ccg ccu uaa aau-5'

There are six possible reading frames in double-stranded DNA. Using the genetic code, translate all possible reading frames of this DNA sequence, keeping in mind the following rules.

In RNA, the T becomes a U, so everywhere you see a T in the sequence, read it as a U.

The genetic code is read in the 5’ to 3’ direction.

Use the single-letter abbreviations for the amino acids because that is what is commonly used by computer programs.

Wiki Hint: if you start your line with a single space character, it will appear in the grey box surrounded by the dashed line as shown above.

Which of the reading frames (if any) of the reading frames you translated is an open reading frame, i.e., does not contain a stop codon?

By convention, the top strand frames are called +1, +2, +3, reading 5' to 3' and the bottom strand frames are called -1, -2, -3, reading 5' to 3'.