Difference between revisions of "Chlamydia trachomatis"
From LMU BioDB 2013
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#*Chlamydiae undergo a biphasic developmental cycle characterized by an infectious cell type known as either an elementary body (EB) and an intracellular replicative form called a reticulate body (RB). Chlamydia was incubated under microaerobic conditions to test the differences in preferred energy source between EB's and RB's. | #*Chlamydiae undergo a biphasic developmental cycle characterized by an infectious cell type known as either an elementary body (EB) and an intracellular replicative form called a reticulate body (RB). Chlamydia was incubated under microaerobic conditions to test the differences in preferred energy source between EB's and RB's. | ||
#*There weren't necessarily a "treatment" group and a "control" group. Referencing figure 4, it can be inferred that EB would be considered the "treatment" group because of the way that the ratio was set up, being EB to RB. In this way the RB would be the "control" group as they are looking at the EB population relative to the RB population. | #*There weren't necessarily a "treatment" group and a "control" group. Referencing figure 4, it can be inferred that EB would be considered the "treatment" group because of the way that the ratio was set up, being EB to RB. In this way the RB would be the "control" group as they are looking at the EB population relative to the RB population. | ||
| − | #*Replicates were performed for the control and treatment | + | #*4 Biological Replicates were performed for the control and treatment; as the article states that "Density gradient-purified EBs and RBs were incubated in quadruplicate in four-well plates...". |
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#Song L, Carlson JH, Whitmire WM, Kari L et al. [http://iai.asm.org/content/81/3/636.long Chlamydia trachomatis plasmid-encoded Pgp4 is a transcriptional regulator of virulence-associated genes.] Infect Immun 2013 Mar;81(3):636-44. | #Song L, Carlson JH, Whitmire WM, Kari L et al. [http://iai.asm.org/content/81/3/636.long Chlamydia trachomatis plasmid-encoded Pgp4 is a transcriptional regulator of virulence-associated genes.] Infect Immun 2013 Mar;81(3):636-44. | ||
Revision as of 18:03, 7 November 2013
Team H(oo)KD:
- Project Manager/Coder: Katrina Sherbina
- Quality Assurance: Hilda Delgadillo
- GenMAPP User: Dillon Williams
Contents |
Annotated Bibliography of Genomics Papers for C. trachomatis
Whole genome sequencing of C. trachomatis
- Journal article describing results of whole genome sequencing:
- Database: PubMed
- Search Terms: Chlamydia trachomatis [MeSH Term] AND genome [Title]
- There were 29 results. Many of the articles that appear using the above search terms have to do with performing whole-genome analysis of C. trachomatis to look at genetic polymorphisms in the bacterium and molecular and genetic characteristics that are observed when the bacterium infects a host.
- Stephens, R.S., Kalman, S., Lammel, C., Fan, J., Marathe, R., Aravind, L., Mitchell, W., Olinger, L., Tatusov, R., Zhao, Q., Koonin, E. V., Davis, R.W. (1998) Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis. Science 282: 754-759. 10.1126/science.282.5389.754.
- The article can be viewed as an HTML here.
- The article can be viewed as a PDF, PDF Version of Genome Paper.
- Use the genome sequencing article you found to perform a prospective search in the ISI Web of Science/Knowledge database.
- How many results did you get?: 872
- Based on the titles and abstracts of the papers, what type of research directions have been taken now that the genome for that organism has been sequenced?: Based on the titles and abstracts found in the results, it seems that research has been primarily in the direction of understanding how the disease functions and assessing the severity of the disease (and how to cure it).
Microarray articles
The EBI ArrayExpress database was used to locate each of the four following microarray articles. The search terms used were Chlamydia trachomatis under 'By organism' while the rest of the drop down menus were not altered.
The following articles appear in order of preference:
- Omsland A, Sager J, Nair V, Sturdevant DE, Hackstadt T. , et al. Developmental stage-specific metabolic and transcriptional activity of Chlamydia trachomatis in an axenic medium
- PDF Version of Paper
- Microarray Data
- Chlamydiae undergo a biphasic developmental cycle characterized by an infectious cell type known as either an elementary body (EB) and an intracellular replicative form called a reticulate body (RB). Chlamydia was incubated under microaerobic conditions to test the differences in preferred energy source between EB's and RB's.
- There weren't necessarily a "treatment" group and a "control" group. Referencing figure 4, it can be inferred that EB would be considered the "treatment" group because of the way that the ratio was set up, being EB to RB. In this way the RB would be the "control" group as they are looking at the EB population relative to the RB population.
- 4 Biological Replicates were performed for the control and treatment; as the article states that "Density gradient-purified EBs and RBs were incubated in quadruplicate in four-well plates...".
Chosen Article for Club Journal
- Opted for paper suggested by professor Dahlquist in post-assignment discussion:
