Stephen Louie Week 4

Transcription and Translation “Taken to the Next Level”

1. Input: cat infA-E.coli-K12.txt | sed "s/cat[at]at/ <-10box> & <\/-10box> /g" | sed "s/................. <-10box>/ <\/-35box> &/g" |sed "s/...... <\/-35box>/ <-35box> &/g" | sed "s/ <-10box> ...... <\/-10box> ...../& <tss> /g" | sed "s/ <tss> ./& <\/tss> /g" | sed "s/gagg/ <rbs> & <\/rbs>\n/g" | sed "2s/atg/ <start_codon> & <\/start_codon> /1" | sed -r "2s/<\/start_codon> .{3}*t[ag][ag]/& <\/stop_codon> /g" | sed "2s/... <\/stop_codon>/ <stop_codon> &/g" | sed "2s/aaaaggt/ <terminator> &/g" | sed "2s/tttt..../& <\/terminator> /2" Output:ttttcaccacaagaatgaatgttttcggcacatttctccccagagtgttataattgcggtcgcagagttggttacgctcattaccccgctgccgataaggaatttttcgcgtcaggtaacgcccatcgtttatctcaccgctcccttatacgttgcgcttttggtgcggcttagccgtgtgttttcggagtaatgtgccgaacctgtttgttgcgatttagcgcgcaaatc <-35box> tttact </-35box> tatttacagaacttcgg <-10box> cattat </-10box> cttgc <tss> c </tss> ggttcaaattacggtagtgatacccca <rbs> gagg </rbs> attag <start_codon> atg </start_codon> gccaaagaagacaatattgaaatgcaaggtaccgttcttgaaacgttgcctaataccatgttccgcgtagagttagaaaacggtcacgtggttactgcacacatctccggtaaaatgcgcaaaaactacatccgcatcctgacgggcgacaaagtgactgttgaactgaccccgtacgacctgagcaaaggccgcattgtcttccgtagtcgc <stop_codon> tga </stop_codon> ttgttttaccgcctgatgggcgaagagaaagaacgagt <terminator> aaaaggtcggtttaaccggcctttttatt </terminator> ttat