Ajvree Week 2

• Write out the complementary strand of DNA below the strand shown and be sure to label the 5’ and 3’ ends of the complementary strand.

5’-cgt-atg-cta-ata-cca-tgt-tcc-gcg-tat-aac-cca-gcc-gcc-agt-tcc-gct-ggc-ggc-att-tta-3’

3'-gca-tac-gat-tat-ggt-aca-agg-cgc-ata-ttg-ggt-cgg-cgg-tca-agg-cga-ccg-ccg-taa-aat-5'

• There are six possible reading frames in double-stranded DNA. Using the genetic code, translate all possible reading frames of this DNA sequence, keeping in mind the following rules.

+1 strand:  R-M-L-I-P-C-S-A-Y-N-P-A-A-S-S-A-G-G-I-L
+2 strand:  V-C-STOP-Y-H-V-P-R-I-T-Q-P-P-V-P-L-A-A-F
+3 strand:  Y-A-N-T-M-F-R-V-STOP-P-S-R-Q-F-R-W-R-H-F

-1 strand:   STOP-N-A-A-S-G-T-G-G-W-V-I-R-G-T-W-STOP-H-T
-2 strand:   

• • In RNA, the T becomes a U, so everywhere you see a T in the sequence, read it as a U.
  • The genetic code is read in the 5’ to 3’ direction.
  • Use the single-letter abbreviations for the amino acids because that is what is commonly used by computer programs.
• Wiki Hint: if you start your line with a single space character, it will appear in the grey box surrounded by the dashed line as shown above.
• Which of the reading frames (if any) of the reading frames you translated is an open reading frame, i.e., does not contain a stop codon?
  • By convention, the top strand frames are called +1, +2, +3, reading 5' to 3' and the bottom strand frames are called -1, -2, -3, reading 5' to 3'.