Lena Week 5

Electronic Journal for Uniprot exercise

• searched for primary accession number: P00533

• This is what the entry showed. It it the main page containing general information about EGFR.

• Clicked on EC 2.7.1.112 link, the link brought up this page. This page can help a person figure out a protein's enzymatic function.

• I navigated back to the original page, then clicked on the Comments heading to see what the comments where. I was directed to this page. The comments page hold useful information that just doesn't fit under any of the other headings.

• Again, I went back to the main page and scrolled down to the cross reference section. I found InterPro and clicked on a link for Graphical View. The page showed a visual for the domains and repeats of the protein.

• I also clicked on EMBL, this is the page that showed up. EMBL showed some basic information about the sequence I clicked on, and showed where repeats occured on the gene.

• I looked at PDB which gave an animation of the protein. I liked this page because it showed you the different domains on the protein.

• For the Pfam Link, I clicked on the graphical view and got this. The image shows the arrangement of Pfam domains on the P00533 sequence.

• I tried out RefSeq, which gave a list of papers where the sequence was referenced. This could be useful in finding out more for a research project.

• Lastly, under the Cross-refs, I tried out GeneID, which gave me this. It gave some general information and showed where the sequence was located genomically.

• Back on the main page, under the heading Ontologies, there were some keywords listed. I clicked on the keyword Tumor Supressor.

• On the main page, under Sequence Annotation, this is what shows up

• I clicked on FASTA on the top right corner to get protein sequence in plain text. Here is the result

>sp|P00533|EGFR_HUMAN Epidermal growth factor receptor OS=Homo sapiens GN=EGFR PE=1 SV=2

MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCEV VLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNMYYENSYALA VLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQWRDIVSSDFLSNMSMDF QNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGRCRGKSPSDCCHNQCAAGC TGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYV VTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFK NCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAF ENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKL FGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCN LLEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVM GENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTNGPKIPSIATGMVGALLLLLVV ALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTPSGEAPNQALLRILKETEFKKIKVLGS GAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASVDNPHVCRLLGI CLTSTVQLITQLMPFGCLLDYVREHKDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAA RNVLVKTPQHVKITDFGLAKLLGAEEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSY GVTVWELMTFGSKPYDGIPASEISSILEKGERLPQPPICTIDVYMIMVKCWMIDADSRPK FRELIIEFSKMARDPQRYLVIQGDERMHLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQ QGFFSSPSTSRTPLLSSLSATSNNSTVACIDRNGLQSCPIKEDSFLQRYSSDPTGALTED SIDDTFLPVPEYINQSVPKRPAGSVQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLN TVQPTCVNSTFDSPAHWAQKGSHQISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRV APQSSEFIGA