Ksherbina Week 5

Katrina Sherbina

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Class Journal

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Individual Journal

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Week 5: Database Wiki

Final Project

Team H(oo)KD Project Page

Journal Club Presentation

Project Individual Journal

Steps to Look Up Human EGFR Protein in the UniProt Database

1. Go to the UniProt Database.
2. Type the UniProt ID P00533 into the Query field in the top middle of the page.
3. Click the Search button.
4. Locate the navigation toolbar, which contains links to different sections in a light blue box with rounded corners that spans the page horizontally.
5. Click on the link Entry information to jump to that section.
   • This section contains information regarding Entry name (for quick identification), Accession Number (unique identifier for the protein), Entry history, Entry status, and Annotation program (these headers appear in a vertical column to the left).
6. In the row of this section containing accession number information, click on the gray icon with two arrows pointing in opposite directions to expand the list of secondary accession numbers for the protein.
7. Scroll back to the top of the page.
8. Type the secondary accession number Q14225 for the protein into the Query filed in the middle of the page.
9. Click the search button.
   • You should be redirected to the page that you were already browsing through.
10. Click on the link Names in the navigation tool bar. This should take you to a section called "Names and origin".
    • This section should contain information regarding Protein names, Gene names, Organism, Taxonomic identifier, and Taxonomic lineage. (these headers appear in a vertical column to the left).
11. Click on the Enzyme Commission number, which is listed after "EC=" in the row designed "Protein names".
    • This will take you to a page in the database ExPASy which gives information regarding what is the biochemical function of human EGFR protein.
12. Return to the previous page in the UniProt database.
13. Click on the link References in the navigation tool bar. This will take you to a section listing various scholarly articles that have discussed the human EGFR protein.
14. Click on the link General annotation in the navigation tool bar.
    • This section contains various information about the protein, such as its function, subcellular location, and post-translational modifications.
15. Click on the link Cross-refs in the navigation tool bar, which will take you to a section titled "Cross-References containing links to entries regarding this protein in different types of databases.
    1. Click on the link X00588, which is the first link that appears under the header "Sequence databases". This link will take you to the EMBL database entry that describes the nucleotide sequence that gives various information regarding the nucleotide sequence codes for the protein.
    2. Go back to the UniProt page and the "Cross-References" section. Scroll down to the header "Family and domain bases" and click on the link IPR000494. This takes you to an entry in the protein database InterPro that describes the EGF receptor, L domain in the protein.