Kmeilak Week 7

Discovery Questions from Chapter 4

5. Choose two genes from Figure 4.6 (PDF of figures on MyLMUConnect) and draw a graph to represent the change in transcription over time.

Media:Microarray_Graph.pdf

6b. Look at Figure 4.7, which depicts the loss of oxygen over time and the transcriptional response of three genes. These data are the ratios of transcription for genes X, Y, and Z during the depletion of oxygen. Using the color scale from Figure 4.6 (bright, medium, dim green, black, dim, medium, or bright red), determine the color for each ratio in Figure 4.7b.

• Gene X (1 hour): black
• Gene X (3 hours): dim red
• Gene X (5 hours): black
• Gene X (9 hours): medium green
• Gene Y (1 hour): black
• Gene Y (3 hours): medium red
• Gene Y (5 hours): dim green
• Gene Y (9 hours): bright green
• Gene Z (1 hour): black
• Gene Z (3 hours): dim red
• Gene Z (5 hours): dim red
• Gene Z (9 hours): dim red

7. Were any of the genes in Figure 4.7b transcribed similarly?

Yes, Gene X and Gene Y begin at 1.0, increase at the 3 hour mark, and then decrease at both the 5 and 9 hour marks.

9. Why would most spots be yellow at the first time point?

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Kmeilak (talk) 21:20, 12 September 2013 (PDT)