Kmeilak Week 8

Overview of Microarray Data Analysis

Electronic Lab Notebook

10/10/13

• Downloaded Merrill Compiled Raw Data file from Sample Microarray Analysis for Vibrio cholerae page
• Saved as Merrell_Compiled_Raw_Data_Vibrio_KM_20131010.xls
• Opened file in excel; created second worksheet and named it scaled_centered
• Copied all data from compiled_raw_data worksheet into scaled_centered worksheet
• Inserted two rows underneath header row (ID, A1, etc)
• Calculated average and standard deviation for each column (i.e. =AVERAGE(B4:B5224); =STDEV(B4:B5224)) by typing function into appropriate labeled row and copying and pasting formulas across all columns.

10/15/13

• Opened

Top 10 Gene Ontology terms

1. macromolecule metabolic process
2. cellular macromolecule metabolic process
3. marcomolecule biosynthesis process
4. biopolymer metabolic process
5. cell projection organization
6. branched chain family amino acid metabolic process
7. amino acid metabolic process
8. cellular amino acid and derivative metabolic process
9. cellular nitrogen compound metabolic process
10. cellular amine metabolic process

Files

• Media:Merrell_Compiled_Raw_Data_Vibrio_KM_20131010.xls
• Media:Merrell_Compiled_Raw_Data_Vibrio_KM_20131010.txt
• Media:Increased_KM-Criterion0-GO.txt
• Media:Merrell_Compiled_Raw_Data_Vibrio_KM_20131010.EX.txt
• Media:Merrell_Compiled_Raw_Data_Vibrio_KM_20131010.gex