Chlamydia trachomatis

Team 1: Chlamydia trachomatis

• Hilda Delgadillo
• Katrina Sherbina
• Dillon Williams

Annotated Bibliography of Genomics Papers for C. trachomatis

Journal article describing results of whole genome sequencing

1. • Database: PubMed
   • Search Terms: Chlamydia trachomatis [MeSH Term] AND genome [Title]
   • Stephens, R.S., Kalman, S., Lammel, C., Fan, J., Marathe, R., Aravind, L.,... Davis, R.W. (1998) Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis. Science 282: 754-759.
2. Use the genome sequencing article you found to perform a prospective search in the ISI Web of Science/Knowledge database.
   • How many results did you get?: 872
   • Based on the titles and abstracts of the papers, what type of research directions have been taken now that the genome for that organism has been sequenced?: Based on the titles and abstracts found in the results, it seems that research has been primarily in the direction of understanding how the disease functions and assessing the severity of the disease (and how to cure it).

Microarray articles

1. Each person needs to find two potential journal articles that refer to public microarray data for your species than are different than what your teammates have found. Thus, each team should find either 6 (for a 3-person team) or 8 (for a 4 person team) articles. If you cannot find all six or eight articles, please let the instructors know right away.
   • The ArrayExpress was used to locate the microarray data.
   • The search terms used were Chlamydia trachomatis under 'By organism' while the rest of the drop down menus were not altered.
   • We were able to get 4 results that match our search exactly, so the data is relevant to the gene expression of Chlamydia trachomatis.
   • Song L, Carlson JH, Whitmire WM, Kari L et al. Chlamydia trachomatis plasmid-encoded Pgp4 is a transcriptional regulator of virulence-associated genes. Infect Immun 2013 Mar;81(3):636-44.
   • [| Chlamydia trachomatis Plasmid-Encoded Pgp4 Is a Transcriptional Regulator of Virulence-Associated Genes]
2. What experiment was performed? What was the "treatment" and what was the "control" in the experiment?
3. Were replicate experiments of the "treatment" and "control" conditions conducted? Were these biological or technical replicates? How many of each?
   • Carlson JH, Whitmire WM, Crane DD, Wicke L et al. The Chlamydia trachomatis plasmid is a transcriptional regulator of chromosomal genes and a virulence factor. Infect Immun 2008 Jun;76(6):2273-83.
   • [| The Chlamydia trachomatis Plasmid Is a Transcriptional Regulator of Chromosomal Genes and a Virulence Factor]
4. What experiment was performed? What was the "treatment" and what was the "control" in the experiment?
5. Were replicate experiments of the "treatment" and "control" conditions conducted? Were these biological or technical replicates? How many of each?