Reference Genome Article

This article was found via the PubMed database and the key terms used were "Leishmania Major [MeSH Terms] AND Genome [Title]"

This search resulted in 30 articles, about 5 seemed relevant but this one was the first which had the entire genome

Citation for paper:

Ivens, AC et al. 2005 Jul 15. The genome of the kinetoplastid parasite, Leishmania major. Science;309(5733):436-42.

Microarray Articles

I typed in "Leishmania Major" in the organism field and "Array assay" in the technology field and filtered down my results.
There were 11 results, 4 seemed relevant
Jean-Michel Ubeda, Danielle Légaré, Frédéric Raymond, Amin Ahmed Ouameur, Sébastien Boisvert, Philippe Rigault, Jacques Corbeil, Michel J Tremblay, Martin Olivier, Barbara Papadopoulou and Marc Ouellette. (2008). Modulation of gene expression in drug resistant Leishmania is associated with gene amplification, gene deletion and chromosome aneuploidy. Genome Biology 9:115.

Searched Science Direct for keywords leishmania, and DNA microarray. There were 40 results on the first page, but the first hit was relevent.
Experiment: This was a microarray experiment comparing genes in leishmania in mice in different environments. The control parasites were initially grown in a neutral media, and the experimental parasites were initially grown in an acidic media where HEPES replaced 4-morpholinoethanesulfonic acid.
Replicates: Hybridization experiments were performed in triplets, so 3 biological replicates.

Citation: Natalia S Akopyants, Robin S Matlib, Elena N Bukanova, Matthew R Smeds, Bernard H Brownstein, Gary D Stormo, Stephen M Beverley, (2004) Expression profiling using random genomic DNA microarrays identifies differentially expressed genes associated with three major developmental stages of the protozoan parasite Leishmania major, Molecular and Biochemical Parasitology, 136: 71-86.

-Lena

I typed in "Leishmania Major" in the organism field and "Array assay" in the technology field and filtered down my results.
There were 11 results, 4 seemed relevant
Rochette, Annie et. al. 2008 May 29.Genome-wide gene expression profiling analysis of Leishmania major and Leishmania infantum developmental stages reveals substantial differences between the two species. BMC Genomics, 9:255.

This article was found using PubMed as well, I searched genome and the species name and found this one especuially interesting because it analyzed the human disease capabilities of the parasite.

Opened browser and navigated to Array Express
Clicked Experiments tab on Array Express home page
Searched for "Leishmania major" in organism tab and "All Arrays" in the arrays tab and "Array assay" by experiment type
This search yielded nine results
Approximately half seemed relevant
Depledge DP, Evans KJ, Ivens AC, Aziz N, Maroof A, et al. (2009) Comparative Expression Profiling of Leishmania: Modulation in Gene Expression between Species and in Different Host Genetic Backgrounds. PLoS Negl Trop Dis 3(7): e476. doi:10.1371/journal.pntd.0000476.

This article was found using PubMed. I searched related citations from the reference paper and got 132 results, this article was one of the top ones. It looked at the genome and which part may be involved in causing disease. I am not sure if the data will be accessible though.

Beverley SM, Akopyants NS, Goyard S, et al. (2002) Putting the Leishmania genome to work: functional genomics by transposon trapping and expression profiling. Philos Trans R Soc Lond B Biol Sci. 2002 Jan 29;357(1417):47-53.

Searched for genomic analysis and leishmania in PubMed. There were 59 results. Many papers were extremely specific, the 14th paper down was this one. It appeared to be a possible resource for information about the leishmania genome. There were 22 results.
Peacock C, Seager K, Harris D, et al. (2007)Comparative genomic analysis of three Leishmania species that cause diverse human disease Genet. 2007 July; 39(7): 839–847.

Experiment: This article does not seem to have a description of exactly what experiments were performed. I am not sure why it was chosen.
Replicates: This article also does not seem to have any mention of replicates.
Citation: Peacock CS, Seeger K, Harris D, Murphy L, Ruiz JC, Quail MA, Peters N, Adlem E, Tivey A, Aslett M, Kerhornou A, Ivens A, Fraser A, Rajandream MA, Carver T, Norbertczak H, Chillingworth T, Hance Z, Jagels K, Moule S, Ormond D, Rutter S, Squares R, Whitehead S, Rabbinowitsch E, Arrowsmith C, White B, Thurston S, Bringaud F, Baldauf SL, Faulconbridge A, Jeffares D, Depledge DP, Oyola SO, Hilley JD, Brito LO, Tosi LR, Barrell B, Cruz AK, Mottram JC, Smith DF, Berriman M, (2007) Comparative genomic analysis of three Leishmania species that cause diverse human disease, National Genetics 7: 839-47.

-Lena