Blitvak: cleaning up/minor edits

2015-09-14T07:52:20Z

cleaning up/minor edits

Blitvak: version 1 of Individual Journal Assignment Week 2 content

2015-09-12T22:56:08Z

version 1 of Individual Journal Assignment Week 2 content

Blitvak: created week 2 individual journal page

2015-09-06T01:39:49Z

created week 2 individual journal page

New page

Under Construction

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 ''Translated Amino Acid Sequence''
   ''Nter''-YANTMFRV''(STOP)''PSRQFRWRHF-''Cter''
 *A reading frame is one of the ways of dividing a strand into triplets (or codons)
-*The are three possible reading frames with the mRNA-like strand, the +1, +2, and +3
+*Stop codons are designated by ''(STOP)''
 *+1 reading frame is from the first base to the last base in a given mRNA-like strand (the entire strand)
 *+2 reading frame excludes the very first base from the reading frame

← Older revision		Revision as of 22:56, 12 September 2015
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−	Under ~~Construction~~	+	==Individual Journal Assignment Week 2==
		+
		+	===Given Strand of DNA===
		+	5’-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta-3’
		+	*Given strand is written from 5’ to 3’; each strand has a complementary antiparallel strand
		+	*The given strand is the mRNA-like strand
		+
		+	===Given Strand and the Complementary Strand===
		+	5’-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta-3’ - ''Given, mRNA-like strand''
		+	3’-gcatacgattatggtacaaggcgcatattgggtcggcggtcaaggcgaccgccgtaaaat-5’ - ''Found, template strand''
		+	*Since the given strand runs from 5’ to 3’, the complementary strand will run from 3’ to 5’
		+	*Complementary strand was found by applying the base pairing of C-G, and A-T
		+	*Under each base letter of the given strand, the corresponding base letter (that forms a base-pair) is written to find the complementary strand
		+	*Since the given strand is 5’ to 3’, it is the strand that is the mRNA-like strand. This is due to the fact that DNA/mRNA must be synthesized from the 5’ to 3’ direction (via the template strand)
		+
		+	===Reading Frames of the mRNA-like strand===
		+	====+1 Reading Frame====
		+	''Transcribed mRNA codons''
		+	5’-cgu aug cua aua cca ugu ucc gcg uau aac cca gcc gcc agu ucc gcu ggc ggc auu uua-3’
		+	''Translated Amino Acid Sequence''
		+	''Nter''-RMLIPCSAYNPAASSAGGIL-''Cter''
		+	====+2 Reading Frame====
		+	''Transcribed mRNA codons''
		+	5’-c gua ugc uaa uac cau guu ccg cgu aua acc cag ccg cca guu ccg cug gcg gca uuu ua-3’
		+	''Translated Amino Acid Sequence''
		+	''Nter''-VC''(STOP)''YHVPRITQPPVPLAAF-''Cter''
		+	====+3 Reading Frame====
		+	''Transcribed mRNA codons''
		+	5’-cg uau gcu aau acc aug uuc cgc gua uaa ccc agc cgc cag uuc cgc ugg cgg cau uuu a-3’
		+	''Translated Amino Acid Sequence''
		+	''Nter''-YANTMFRV''(STOP)''PSRQFRWRHF-''Cter''
		+	*A reading frame is one of the ways of dividing a strand into triplets (or codons)
		+	*The are three possible reading frames with the mRNA-like strand, the +1, +2, and +3
		+	*+1 reading frame is from the first base to the last base in a given mRNA-like strand (the entire strand)
		+	*+2 reading frame excludes the very first base from the reading frame
		+	*+3 reading frame excludes the first two bases from the reading frame
		+	*It is helpful to group the sequences into triplets and exclude incomplete triplets
		+	*Use an [https://en.wikipedia.org/wiki/Genetic_code#RNA_codon_table: RNA Codon Table] to find the amino acids that correspond to the codons (triplets) in mRNA
		+	*Different reading frames lead to different sequences of amino acids
		+	*All reading frames are from 5’ to 3’
		+
		+	===Reading Frames of the template strand===
		+	====-1 Reading Frame====
		+	''Transcribed mRNA codons''
		+	5’-uaa aau gcc gcc agc gga acu ggc ggc ugg guu aua cgc gga aca ugg uau uag cau acg-3’
		+	''Translated Amino Acid Sequence''
		+	''Nter''-''(STOP)''NAASGTGGWVIRGTWY''(STOP)''HT-''Cter''
		+	====-2 Reading Frame====
		+	''Transcribed mRNA codons''
		+	5’-u aaa aug ccg cca gcg gaa cug gcg gcu ggg uua uac gcg gaa cau ggu auu agc aua cg-3’
		+	''Translated Amino Acid Sequence''
		+	''Nter''-KMPPAELAAGLYAEHGISI-''Cter''
		+	====-3 Reading Frame====
		+	''Transcribed mRNA codons''
		+	5’-ua aaa ugc cgc cag cgg aac ugg cgg cug ggu uau acg cgg aac aug gua uua gca uac g-3’
		+	''Translated Amino Acid Sequence''
		+	''Nter''-KCRQRNWRLGYTRNMVLAY-''Cter''
		+	*Finding reading frames is the same for the template strand as it is for the mRNA-like strand, but each is designated as -1, -2, and -3 respectively
		+	*Since there are also 3 possible reading frames for the template strand, there are 6 total reading frames in double-stranded DNA
		+	*Since the complementary template strand is 3’ to 5’, it must be reversed so that it is 5’ to 3’ (prior to finding its reading frames)
		+
		+	===Open Reading Frames===
		+
		+	*The +1, -2, and -3 reading frames are all open reading frames since they all contain no stop codons in their mRNA sequences
		+
		+
		+	-----
		+
		+	{{Template:blitvak}}

Blitvak Week 2 - Revision history

Blitvak: cleaning up/minor edits

Blitvak: version 1 of Individual Journal Assignment Week 2 content

Blitvak: created week 2 individual journal page