Vpachec3 Week 10 - Revision history

Vpachec3: /* Final Paper Choice and the Annotated Bibliography */ added second annotated bib article

2015-11-10T07:10:03Z

‎Final Paper Choice and the Annotated Bibliography: added second annotated bib article

2015-11-10T06:31:12Z

‎Final Paper Choice and the Annotated Bibliography: edited last answer

2015-11-10T06:18:42Z

‎Final Paper Choice and the Annotated Bibliography: added answer to final question

2015-11-09T19:19:02Z

‎Final Paper Choice and the Annotated Bibliography: fixed bolding

2015-11-09T19:17:58Z

‎Final Paper Choice and the Annotated Bibliography: Added answer to second to last question

@@ Line 160: / Line 160: @@
 *#**# What experiment was performed?  What was the "treatment" and what was the "control" in the experiment? '''B. cenocepacia J2315 cells were treated by exposure to high levels of H2O2, NaOCl or chlorhexidine. In addition differences in gene expression were examined between untreated B. cenocepacia sessile cells and B. cenocepacia planktonic cells.'''
 *#**# Were replicate experiments of the "treatment" and "control" conditions conducted?  Were these biological or technical replicates?  How many of each? '''There were 20 assays.Hybridizations for all experimental conditions were performed in 4 replicates.'''
 ==Links==

@@ Line 159: / Line 159: @@
 *#** For each of the microarray articles/datasets, answer the following:
 *#**# What experiment was performed?  What was the "treatment" and what was the "control" in the experiment? '''B. cenocepacia J2315 cells were treated by exposure to high levels of H2O2, NaOCl or chlorhexidine. In addition differences in gene expression were examined between untreated B. cenocepacia sessile cells and B. cenocepacia planktonic cells.'''
-*#**# Were replicate experiments of the "treatment" and "control" conditions conducted?  Were these biological or technical replicates?  How many of each? '''There were 20 assays which means that there were  10 biological replicates.'''
+*#**# Were replicate experiments of the "treatment" and "control" conditions conducted?  Were these biological or technical replicates?  How many of each? '''There were 20 assays.Hybridizations for all experimental conditions were performed in 4 replicates.'''
 ==Links==

@@ Line 159: / Line 159: @@
 *#** For each of the microarray articles/datasets, answer the following:
 *#**# What experiment was performed?  What was the "treatment" and what was the "control" in the experiment? '''B. cenocepacia J2315 cells were treated by exposure to high levels of H2O2, NaOCl or chlorhexidine. In addition differences in gene expression were examined between untreated B. cenocepacia sessile cells and B. cenocepacia planktonic cells.'''
-*#**# Were replicate experiments of the "treatment" and "control" conditions conducted?  Were these biological or technical replicates?  How many of each?
+*#**# Were replicate experiments of the "treatment" and "control" conditions conducted?  Were these biological or technical replicates?  How many of each? '''There were 20 assays which means that there were  10 biological replicates.'''
 ==Links==

@@ Line 158: / Line 158: @@
 *#* In addition, you must also link to the web site where the microarray data resides.[ https://www.ebi.ac.uk/arrayexpress/experiments/E-MEXP-2502/?keywords=Burkholderia+cenocepacia+J2315&organism=&exptype%5B%5D=%22rna+assay%22&exptype%5B%5D=%22array+assay%22&array=]
 *#** For each of the microarray articles/datasets, answer the following:
-*#**# What experiment was performed?  What was the "treatment" and what was the "control" in the experiment? '''B. cenocepacia J2315 cells
+*#**# What experiment was performed?  What was the "treatment" and what was the "control" in the experiment? '''B. cenocepacia J2315 cells were treated by exposure to high levels of H2O2, NaOCl or chlorhexidine. In addition differences in gene expression were examined between untreated B. cenocepacia sessile cells and B. cenocepacia planktonic cells.'''
 were treated by exposure to high levels of H2O2, NaOCl or chlorhexidine. In addition differences in gene expression were examined between untreated B. cenocepacia sessile cells and B. cenocepacia planktonic cells.'''
 *#**# Were replicate experiments of the "treatment" and "control" conditions conducted?  Were these biological or technical replicates?  How many of each?

@@ Line 158: / Line 158: @@
 *#* In addition, you must also link to the web site where the microarray data resides.[ https://www.ebi.ac.uk/arrayexpress/experiments/E-MEXP-2502/?keywords=Burkholderia+cenocepacia+J2315&organism=&exptype%5B%5D=%22rna+assay%22&exptype%5B%5D=%22array+assay%22&array=]
 *#** For each of the microarray articles/datasets, answer the following:
-*#**# What experiment was performed?  What was the "treatment" and what was the "control" in the experiment?
+*#**# What experiment was performed?  What was the "treatment" and what was the "control" in the experiment? '''B. cenocepacia J2315 cells
 *#**# Were replicate experiments of the "treatment" and "control" conditions conducted?  Were these biological or technical replicates?  How many of each?