Difference between revisions of "Vpachec3 Week 2"
From LMU BioDB 2015
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| + | === The Genetic Code === | ||
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| + | * Write out the complementary strand of DNA below the strand shown and be sure to label the 5’ and 3’ ends of the complementary strand. | ||
| + | |||
| + | 5’-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta-3’ | ||
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| + | * There are six possible reading frames in double-stranded DNA. Using the [http://en.wikipedia.org/wiki/Genetic_code#RNA_codon_table genetic code], translate all possible reading frames of this DNA sequence, keeping in mind the following rules. | ||
| + | ** In RNA, the T becomes a U, so everywhere you see a T in the sequence, read it as a U. | ||
| + | ** The genetic code is read in the 5’ to 3’ direction. | ||
| + | ** Use the single-letter abbreviations for the amino acids because that is what is commonly used by computer programs. | ||
| + | * Wiki Hint: if you start your line with a single space character, it will appear in the grey box surrounded by the dashed line as shown above. | ||
| + | * Which of the reading frames (if any) of the reading frames you translated is an ''open reading frame'', i.e., does not contain a stop codon? | ||
| + | ** By convention, the top strand frames are called +1, +2, +3, reading 5' to 3' and the bottom strand frames are called -1, -2, -3, reading 5' to 3'. | ||
| + | |||
| + | ==Complementary Strand== | ||
| + | 3’-gcatacgattatggtacaagcgcatattgggtcggcggtcaaggcgaccgccgtaaaat-5’ | ||
| + | |||
| + | ==RNA== | ||
| + | 5’-cguaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuua-3’ | ||
==Links== | ==Links== | ||
Revision as of 01:39, 15 September 2015
The Genetic Code
- Write out the complementary strand of DNA below the strand shown and be sure to label the 5’ and 3’ ends of the complementary strand.
5’-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta-3’
- There are six possible reading frames in double-stranded DNA. Using the genetic code, translate all possible reading frames of this DNA sequence, keeping in mind the following rules.
- In RNA, the T becomes a U, so everywhere you see a T in the sequence, read it as a U.
- The genetic code is read in the 5’ to 3’ direction.
- Use the single-letter abbreviations for the amino acids because that is what is commonly used by computer programs.
- Wiki Hint: if you start your line with a single space character, it will appear in the grey box surrounded by the dashed line as shown above.
- Which of the reading frames (if any) of the reading frames you translated is an open reading frame, i.e., does not contain a stop codon?
- By convention, the top strand frames are called +1, +2, +3, reading 5' to 3' and the bottom strand frames are called -1, -2, -3, reading 5' to 3'.
Complementary Strand
3’-gcatacgattatggtacaagcgcatattgggtcggcggtcaaggcgaccgccgtaaaat-5’
RNA
5’-cguaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuua-3’
