Difference between revisions of "Vpachec3 Week 2"

Revision as of 03:03, 15 September 2015

The Genetic Code

• Write out the complementary strand of DNA below the strand shown and be sure to label the 5’ and 3’ ends of the complementary strand.

5’-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta-3’

• There are six possible reading frames in double-stranded DNA. Using the genetic code, translate all possible reading frames of this DNA sequence, keeping in mind the following rules.
  • In RNA, the T becomes a U, so everywhere you see a T in the sequence, read it as a U.
  • The genetic code is read in the 5’ to 3’ direction.
  • Use the single-letter abbreviations for the amino acids because that is what is commonly used by computer programs.
• Wiki Hint: if you start your line with a single space character, it will appear in the grey box surrounded by the dashed line as shown above.
• Which of the reading frames (if any) of the reading frames you translated is an open reading frame, i.e., does not contain a stop codon?
  • By convention, the top strand frames are called +1, +2, +3, reading 5' to 3' and the bottom strand frames are called -1, -2, -3, reading 5' to 3'.

Complementary Strand

3’-gcatacgattatggtacaagcgcatattgggtcggcggtcaaggcgaccgccgtaaaat-5’

RNA +1

5’-cguaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuua-3’

Amino Acid sequence +1

N-ter- R-M-L-I-P-C-S-A-Y-N-P-A-A-S-S-A-G-G-I-L- C-ter

RNA +2

5’-guaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuua-3’

Amino Acid sequence +2

N-ter-V-C-STOP-Y-H-V-P-R-I-T-Q-P-P-V-P-L-A-A-F-C-ter

RNA +3

5’-uaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuua-3’

Amino Acid sequence +3

N-ter-Y-A-N-T-M-F-R-V-STOP-P-S-R-Q-F-R-W-R-H-F-C-ter

RNA -1

5’-uaaaaugccgccagcggaacuggcggcuggguuauacgcggaacaugguauuagcauacg-3’

Amino Acid sequence -1

N-ter- - C-ter

Links

Vpachec3 User Page