* According to the week 4 assignment, the terminator hairpin loop starts with aaaaggt, where the t will end up binding with a g. This hairpin loop bends around and connects to itself, and an additional 4 nucleotides exist after the hairpin loop. Since I know the hairpin binds with itself, I know that the complement of this sequence will exist in reverse, only it will begin with a g instead of an a, as the t connects to a g. So I know that gcctttt will also exist in the terminator, and the 4 nucleotides following that sequence will also be included. Using everything I'd learned up to this point, I was able to easily construct a command that would allow me to easily create the tag for the terminator despite the added complexity. The command and final output is as follows: | * According to the week 4 assignment, the terminator hairpin loop starts with aaaaggt, where the t will end up binding with a g. This hairpin loop bends around and connects to itself, and an additional 4 nucleotides exist after the hairpin loop. Since I know the hairpin binds with itself, I know that the complement of this sequence will exist in reverse, only it will begin with a g instead of an a, as the t connects to a g. So I know that gcctttt will also exist in the terminator, and the 4 nucleotides following that sequence will also be included. Using everything I'd learned up to this point, I was able to easily construct a command that would allow me to easily create the tag for the terminator despite the added complexity. The command and final output is as follows: |