**I began by identifying the minus 35 box. Using the tips from the assignment, I used the command <code>grep "tt[gt]ac[at]" infA-E.coli-K12.txt</code> to identify sequences matches for the minus 35 box. There were two matches.

**I began by identifying the minus 35 box. Using the tips from the assignment, I used the command <code>grep "tt[gt]ac[at]" infA-E.coli-K12.txt</code> to identify sequences matches for the minus 35 box. There were two matches.

**To identify which match was the the minus 35 box, I performed the command <code>grep "[ct]at[at]at" infA-E.coli-K12.txt</code> to identify possible minus 10 box matches. Because we know the minus 10 box match must occur 17 nucleotides after the minus 35 box, only one pair of matches for the minus 35 & 10 boxes was possible. Although this process does require some subjective analysis, we discussed in class that this was permissible in this case to initiate the tagging process.

**To identify which match was the the minus 35 box, I performed the command <code>grep "[ct]at[at]at" infA-E.coli-K12.txt</code> to identify possible minus 10 box matches. Because we know the minus 10 box match must occur 17 nucleotides after the minus 35 box, only one pair of matches for the minus 35 & 10 boxes was possible. Although this process does require some subjective analysis, we discussed in class that this was permissible in this case to initiate the tagging process.