**#A new line was started after the tag to make it simpler to perform subsequent operations on the sequence.

**#A new line was started after the tag to make it simpler to perform subsequent operations on the sequence.

**#The command is as follows:

**#The command is as follows:

  sed "s/tt[gt]ac[at]/<minus35box>&<\/minux35box>\n/1" infA-E.coli-K12.txt

sed "s/tt[gt]ac[at]/<minus35box>&<\/minux35box>\n/1" infA-E.coli-K12.txt

*'''Minus 10 Box'''

*'''Minus 10 Box'''

**After identifying the minus 35 box, I broke the nucleotide sequence up into two lines as detailed above. Because we know that the minus 10 box exists 17 nucleotides after the minus 35 box and is 6 nucleotides long, this meant that the minus 10 box presumably would always consist of characters 18-23 in line 2 of the code.

**After identifying the minus 35 box, I broke the nucleotide sequence up into two lines as detailed above. Because we know that the minus 10 box exists 17 nucleotides after the minus 35 box and is 6 nucleotides long, this meant that the minus 10 box presumably would always consist of characters 18-23 in line 2 of the code. I verified this for the sequence present in ''infA-E.coli-K12.txt'' by checking that the sequence 17 nucleotides after the tagged minus 35 box did indeed match the minus 10 box sequence.

**To automate the tagging of the minus 10 box, I added a series of two separate sed commands to the pipe that would subjectively find this sequence based on its location in line 2.

**To automate the tagging of the minus 10 box, I added a series of two separate sed commands to the pipe that would subjectively find this sequence based on its location in line 2.

**#I wrote the first sed command to add the tag <code><minus10box></code> after the 17th character of the second line.

**#I wrote the first sed command to add the tag <code><minus10box></code> after the 17th character of the second line, taking advantage of the <code>sed -r</code> repetition shortcut.

**#I wrote the second sed command to add the tag <code></minus10box></code> 6 characters after the first minus 10 box tag.

**#I wrote the second sed command to add the tag <code></minus10box></code> 6 characters after the first minus 10 box tag.

**#With the addition of these two commands, my command sequence was as follows:

**#With the addition of these two commands, my command sequence was as follows: