#*Most spots would be yellow at the first time point because there is no change in gene expression, yet (equal expression in the, red, experimental group and the, green, control group); the gene regulatory response to the different conditions is not immediate (takes some time for the regulatory processes to react to the different conditions). Technically speaking, the yellow color is the result of there being equal (or near equal) amounts of green and red fluorescing DNA. Biologically speaking, the reason that the experiment resulted in mostly yellow spots would be because the gene expression for most genes did not change; the gene expression of only a few genes changed because that is all that is necessary for/related to the new conditions.   

#*Most spots would be yellow at the first time point because there is no change in gene expression, yet (equal expression in the, red, experimental group and the, green, control group); the gene regulatory response to the different conditions is not immediate (takes some time for the regulatory processes to react to the different conditions). Technically speaking, the yellow color is the result of there being equal (or near equal) amounts of green and red fluorescing DNA. Biologically speaking, the reason that the experiment resulted in mostly yellow spots would be because the gene expression for most genes did not change; the gene expression of only a few genes changed because that is all that is necessary for/related to the new conditions.   

#(Question 10, p. 118) Go to the Saccharomyces Genome Database and search for the gene TEF4; you will see it is involved in translation. Look at the time point labeled OD 3.7 in Figure 4.12, and find the TEF4 spot. Over the course of this experiment, was TEF4 induced or repressed? Hypothesize why TEF4’s change in expression was part of the cell’s response to a reduction in available glucose (i.e., the only available food).

#(Question 10, p. 118) Go to the Saccharomyces Genome Database and search for the gene TEF4; you will see it is involved in translation. Look at the time point labeled OD 3.7 in Figure 4.12, and find the TEF4 spot. Over the course of this experiment, was TEF4 induced or repressed? Hypothesize why TEF4’s change in expression was part of the cell’s response to a reduction in available glucose (i.e., the only available food).