#*[[file:TABLE1_WK7_BL.png]]

#*[[file:TABLE1_WK7_BL.png]]

#(Question 7, p. 110) Were any of the genes in Figure 4.7b transcribed similarly? If so, which ones were transcribed similarly to which ones?

#(Question 7, p. 110) Were any of the genes in Figure 4.7b transcribed similarly? If so, which ones were transcribed similarly to which ones?

#*Gene X and Gene Y were somewhat similarly transcribed since they were both first up-regulated (at 3 hours) and then later heavily down-regulated (at 9 hours). At one hour, there existed no changes for both genes; at 5 hours, gene Y was slightly down-regulated while gene X remained the same. Compared to gene X, gene Y was also showed a higher degree of up-regulation and down-regulation.  

#*Gene X and Gene Y were somewhat similarly transcribed since they were both first up-regulated (at 3 hours) and then later heavily down-regulated (at 9 hours). At one hour, there existed no changes for both genes; at 5 hours, gene Y was slightly down-regulated while gene X remained the same. Compared to gene X, gene Y also showed a higher degree of up-regulation and down-regulation.  

#(Question 9, p. 118) Why would most spots be yellow at the first time point? I.e., what is the technical reason that spots show up as yellow - where does the yellow color come from? And, what would be the biological reason that the experiment resulted in most spots being yellow?

#(Question 9, p. 118) Why would most spots be yellow at the first time point? I.e., what is the technical reason that spots show up as yellow - where does the yellow color come from? And, what would be the biological reason that the experiment resulted in most spots being yellow?

#*Most spots would be yellow at the first time point because there is no change in gene expression, yet (equal expression in the, red, experimental group and the, green, control group); the gene regulatory response to the different conditions is not immediate (takes some time for the regulatory processes to react to the different conditions). Technically speaking, the yellow color is the result of there being equal (or near equal) amounts of green and red fluorescing DNA. Biologically speaking, the reason that the experiment resulted in mostly yellow spots would be because the gene expression for most genes did not change; the gene expression of only a few genes changed because that is all that is necessary for/related to the new conditions.

#*Most spots would be yellow at the first time point because there is no change in gene expression, yet (equal expression in the, red, experimental group and the, green, control group); the gene regulatory response to the different conditions is not immediate (takes some time for the regulatory processes to react to the different conditions). Technically speaking, the yellow color is the result of there being equal (or near equal) amounts of green and red fluorescing DNA. Biologically speaking, the reason that the experiment resulted in mostly yellow spots would be because the gene expression for most genes did not change; the gene expression of only a few genes changed because that is all that is necessary for/related to the new conditions. If certain spots are yellow, then that indicates that the current conditions are not different enough to warrant changes in transcription.

#(Question 10, p. 118) Go to the Saccharomyces Genome Database and search for the gene TEF4; you will see it is involved in translation. Look at the time point labeled OD 3.7 in Figure 4.12, and find the TEF4 spot. Over the course of this experiment, was TEF4 induced or repressed? Hypothesize why TEF4’s change in expression was part of the cell’s response to a reduction in available glucose (i.e., the only available food).

#(Question 10, p. 118) Go to the Saccharomyces Genome Database and search for the gene TEF4; you will see it is involved in translation. Look at the time point labeled OD 3.7 in Figure 4.12, and find the TEF4 spot. Over the course of this experiment, was TEF4 induced or repressed? Hypothesize why TEF4’s change in expression was part of the cell’s response to a reduction in available glucose (i.e., the only available food).