Difference between revisions of "Blitvak Week 8"

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(10/22 info (class work))
(added top 10 GO terms)
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==Week 8 Individual Journal==
 
==Week 8 Individual Journal==
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===DNA Microarray Analysis part 1===
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*[http://www.openwetware.org/images/d/d6/Merrell_Compiled_Raw_Data_Vibrio.xls Merrell_Compiled_Raw_Data_Vibrio.xls] was downloaded and opened with Excel
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*
  
 
*[[Media:Merrell_Compiled_Raw_Data_Vibrio_BL_20151015.xls|Modified Excel File/Data]]  
 
*[[Media:Merrell_Compiled_Raw_Data_Vibrio_BL_20151015.xls|Modified Excel File/Data]]  
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*[[Media:Merrell_Compiled_Raw_Data_Vibrio_BL_20151015.txt|For GenMAPP]]
 
*[[Media:Merrell_Compiled_Raw_Data_Vibrio_BL_20151015.txt|For GenMAPP]]
  
*[http://sourceforge.net/projects/xmlpipedb/files/V.%20cholerae%20Gene%20Database/V.%20cholerae%2020090622/Vc-Std_External_20090622.zip/download Working with: 2009 Vibrio Cholera database]
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[http://sourceforge.net/projects/xmlpipedb/files/V.%20cholerae%20Gene%20Database/V.%20cholerae%2020090622/Vc-Std_External_20090622.zip/download Working with: 2009 Vibrio Cholera database]
  
 
*772 errors were detected in the raw data by genMAPP using the 2009 database
 
*772 errors were detected in the raw data by genMAPP using the 2009 database
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==Results of 10/22 Work Session==
 
==Results of 10/22 Work Session==
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*'''Top 10 Ranked GO Terms'''
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#macromolecule metabolic process
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#localization
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#transporter activity
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#cellular macromolecule metabolic process
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#transport
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#establishment of localization
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#cell projection organization
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#cellular biopolymer metabolic process
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#macromolecule biosynthetic process
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#biopolymer metabolic process
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*Analysis of the .EX.txt file produced by GenMAPP, via Excel, revealed that the 772 errors were: ''Gene not found in OrderedLocusNames or any related system''; this suggests that the 2009 database did not   
 
*Analysis of the .EX.txt file produced by GenMAPP, via Excel, revealed that the 772 errors were: ''Gene not found in OrderedLocusNames or any related system''; this suggests that the 2009 database did not   

Revision as of 22:35, 22 October 2015

Week 8 Individual Journal

DNA Microarray Analysis part 1

Working with: 2009 Vibrio Cholera database

  • 772 errors were detected in the raw data by genMAPP using the 2009 database
  • 121 errors were detected in the raw data by genMAPP using the 2010 database by my partner, Anindita V.

Results of 10/20 Work Session

.gex file for GenMAPP

Results of 10/22 Work Session

  • Top 10 Ranked GO Terms
  1. macromolecule metabolic process
  2. localization
  3. transporter activity
  4. cellular macromolecule metabolic process
  5. transport
  6. establishment of localization
  7. cell projection organization
  8. cellular biopolymer metabolic process
  9. macromolecule biosynthetic process
  10. biopolymer metabolic process


  • Analysis of the .EX.txt file produced by GenMAPP, via Excel, revealed that the 772 errors were: Gene not found in OrderedLocusNames or any related system; this suggests that the 2009 database did not


increased expression


Brandon Litvak
BIOL 367, Fall 2015

Weekly Assignments Individual Journal Pages Shared Journal Pages