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| *** '''It is likely that you will have a different number of errors than your partner who is using a different version of the ''Vibrio cholerae'' Gene Database. Which of you has more errors? Why do you think that is?''' | | *** '''It is likely that you will have a different number of errors than your partner who is using a different version of the ''Vibrio cholerae'' Gene Database. Which of you has more errors? Why do you think that is?''' |
| ****With the 2009 Vibrio cholerae gene database loaded into GenMAPP, I encountered 772 errors when converting the analyzed Merrell et al. microarray data. When converting the exact same data with the 2010 Vibrio cholera gene database loaded into GenMapp, Veronica encountered 121 errors. All error messages for both of us were the same: "Gene not found in OrderedLocusNames or any related system". Therefore, I had more errors. I believe this occured because the 2009 version of the Vibrio cholerae database was less developed/complete than the more recent 2010 version. Thus, it likely had less Gene listings, and therefore less of the Ordered Locus Names used by Merrell matched to the database. | | ****With the 2009 Vibrio cholerae gene database loaded into GenMAPP, I encountered 772 errors when converting the analyzed Merrell et al. microarray data. When converting the exact same data with the 2010 Vibrio cholera gene database loaded into GenMapp, Veronica encountered 121 errors. All error messages for both of us were the same: "Gene not found in OrderedLocusNames or any related system". Therefore, I had more errors. I believe this occured because the 2009 version of the Vibrio cholerae database was less developed/complete than the more recent 2010 version. Thus, it likely had less Gene listings, and therefore less of the Ordered Locus Names used by Merrell matched to the database. |
− | *** '''Upload your exceptions file: <code>EX.txt</code> to your wiki page.
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− | * Customize the new Expression Dataset by creating new Color Sets which contain the instructions to GenMAPP for displaying data on MAPPs.
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− | ** Color Sets contain the instructions to GenMAPP for displaying data from an Expression Dataset on MAPPs. Create a Color Set by filling in the following different fields in the Color Set area of the Expression Dataset Manager: a name for the Color Set, the gene value, and the criteria that determine how a gene object is colored on the MAPP. Enter a name in the Color Set Name field that is 20 characters or fewer.
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− | ** The Gene Value is the data displayed next to the gene box on a MAPP. Select the column of data to be used as the Gene Value from the drop down list or select [none]. We will use "Avg_LogFC_all" for the Vibrio dataset you just created.
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− | ** Activate the Criteria Builder by clicking the New button.
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− | ** Enter a name for the criterion in the Label in Legend field.
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− | ** Choose a color for the criterion by left-clicking on the Color box. Choose a color from the Color window that appears and click OK.
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− | ** State the criterion for color-coding a gene in the Criterion field.
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− | *** A criterion is stated with relationships such as "this column greater than this value" or "that column less than or equal to that value". Individual relationships can be combined using as many ANDs and ORs as needed. A typical relationship is
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− | [ColumnName] RelationalOperator Value
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− | ::with the column name always enclosed in brackets and character values enclosed in single quotes. For example:
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− | [Fold Change] >= 2
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− | [p value] < 0.05
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− | [Quality] = 'high'
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− | ::This is the equivalent to queries that you performed on the command line when working with the PostgreSQL movie database. GenMAPP is using a graphical user interface (GUI) to help the user format the queries correctly. The easiest and safest way to create criteria is by choosing items from the Columns and Ops (operators) lists shown in the Criteria Builder. The Columns list contains all of the column headings from your Expression Dataset. To choose a column from the list, click on the column heading. It will appear at the location of the cursor in the Criterion box. The Criteria Builder surrounds the column names with brackets.
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| | | |
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Backtrace:
#0 /apps/xmlpipedb/biodb/fall2015/includes/diff/DairikiDiff.php(544): DiffEngine->diag()
#1 /apps/xmlpipedb/biodb/fall2015/includes/diff/DairikiDiff.php(344): DiffEngine->compareSeq()
#2 /apps/xmlpipedb/biodb/fall2015/includes/diff/DairikiDiff.php(227): DiffEngine->diffLocal()
#3 /apps/xmlpipedb/biodb/fall2015/includes/diff/DairikiDiff.php(721): DiffEngine->diff()
#4 /apps/xmlpipedb/biodb/fall2015/includes/diff/DairikiDiff.php(859): Diff->__construct()
#5 /apps/xmlpipedb/biodb/fall2015/includes/diff/DairikiDiff.php(980): MappedDiff->__construct()
#6 /apps/xmlpipedb/biodb/fall2015/includes/diff/TableDiffFormatter.php(194): WordLevelDiff->__construct()
#7 /apps/xmlpipedb/biodb/fall2015/includes/diff/DiffFormatter.php(140): TableDiffFormatter->changed()
#8 /apps/xmlpipedb/biodb/fall2015/includes/diff/DiffFormatter.php(82): DiffFormatter->block()
#9 /apps/xmlpipedb/biodb/fall2015/includes/diff/DifferenceEngine.php(888): DiffFormatter->format()
#10 /apps/xmlpipedb/biodb/fall2015/includes/diff/DifferenceEngine.php(802): DifferenceEngine->generateTextDiffBody()
#11 /apps/xmlpipedb/biodb/fall2015/includes/diff/DifferenceEngine.php(733): DifferenceEngine->generateContentDiffBody()
#12 /apps/xmlpipedb/biodb/fall2015/includes/diff/DifferenceEngine.php(662): DifferenceEngine->getDiffBody()
#13 /apps/xmlpipedb/biodb/fall2015/includes/diff/DifferenceEngine.php(632): DifferenceEngine->getDiff()
#14 /apps/xmlpipedb/biodb/fall2015/includes/diff/DifferenceEngine.php(453): DifferenceEngine->showDiff()
#15 /apps/xmlpipedb/biodb/fall2015/includes/page/Article.php(795): DifferenceEngine->showDiffPage()
#16 /apps/xmlpipedb/biodb/fall2015/includes/page/Article.php(506): Article->showDiffPage()
#17 /apps/xmlpipedb/biodb/fall2015/includes/actions/ViewAction.php(44): Article->view()
#18 /apps/xmlpipedb/biodb/fall2015/includes/MediaWiki.php(395): ViewAction->show()
#19 /apps/xmlpipedb/biodb/fall2015/includes/MediaWiki.php(273): MediaWiki->performAction()
#20 /apps/xmlpipedb/biodb/fall2015/includes/MediaWiki.php(566): MediaWiki->performRequest()
#21 /apps/xmlpipedb/biodb/fall2015/includes/MediaWiki.php(414): MediaWiki->main()
#22 /apps/xmlpipedb/biodb/fall2015/index.php(44): MediaWiki->run()
#23 {main}