*Dye swaps - unable to find evidence of this in the paper

*Dye swaps - unable to find evidence of this in the paper

==Vocab==

==Vocab Definitions==

#Putative: entity or concept that is based on what is generally accepted or inferred even without direct proof

#Putative: entity or concept that is based on what is generally accepted or inferred even without direct proof

#*[[http://www.biology-online.org/dictionary/Putative Citation]]

#*[[http://www.biology-online.org/dictionary/Putative Citation]]

#2-2'-dipyridyl: chelator of iron

#2-2'-dipyridyl: chelator of iron

#*[[http://www.scbt.com/datasheet-206502-2-2-dipyridyl.html Citation]]

#*[[http://www.scbt.com/datasheet-206502-2-2-dipyridyl.html Citation]]

==Outline==

==Outline==

*What were the methods used in the study?  

*What were the methods used in the study?  

**First, the researchers amplified a strand of wild-type S. oneidensis MR-1 using PCR. They confirmed that they had the desired sequence using DNA sequencing. (14)

**First, the researchers amplified a strand of wild-type S. oneidensis MR-1 using PCR. They confirmed that they had the desired sequence using DNA sequencing. (14)

**To test the uptake of iron in aerobic versus anaerobic conditions, the MR-1 was first grown to mid-log phase and diluted. They then created diluted solutions of 2,2’-dipyridyl in 80 M, 160 M, 240 M, and 320 M concentrations. This was done in triplicates for each condition. Next, ferrous sulfate solution was added to replete the iron. They used a Luria-Bertani medium at 30 C for the aerobic culturing and supplemented this medium with 10mM lactate and an electron acceptor for the anaerobic culturing. (14)

**To test the uptake of iron in aerobic versus anaerobic conditions, the MR-1 was first grown to mid-log phase and diluted. They then created diluted solutions of 2,2’-dipyridyl in 80 micro-M, 160 micro-M, 240 micro-M, and 320 micro-M concentrations. This was done in triplicates for each condition. Next, ferrous sulfate solution was added to replete the iron. They used a Luria-Bertani medium at 30 C for the aerobic culturing and supplemented this medium with 10mM lactate and an electron acceptor for the anaerobic culturing. (14)

**To test the iron reduction rate, cells were grown anaerobically to mid-log phase in the LB medium along with 10mM fumarate and 10mM lactate. They were transferred to a solution of 5mL LB medium, 10mM lactate, and 10mM Fe(III) dioxide. They then performed the ferrozine assay. (14).

**To test the iron reduction rate, cells were grown anaerobically to mid-log phase in the LB medium along with 10mM fumarate and 10mM lactate. They were transferred to a solution of 5mL LB medium, 10mM lactate, and 10mM Fe(III) dioxide. They then performed the ferrozine assay. (14).